Kolodziejczyk E, Serrant P, Fernandez-Graf M R
Section of Microscopy, Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
J Neurosci Methods. 1990 Mar;31(3):183-6. doi: 10.1016/0165-0270(90)90108-r.
A rotary wire saw is faster and simplifies markedly the slicing of soft and hard tissues without apparent loss of quality. Two applications for the Golgi-Cox method (using the rotary wire saw) are described: one eliminates specimen freezing and embedding while the other uses LR-White instead of celloidin, reducing preparation time. Sections of 20 microns to several mm thick can be obtained.
旋转线锯速度更快，能显著简化软组织和硬组织切片过程，且质量无明显损失。本文描述了高尔基体 - 考克斯法（使用旋转线锯）的两种应用：一种无需对标本进行冷冻和包埋，另一种使用LR - 白色树脂而非火棉胶，可缩短制备时间。切片厚度可达20微米至几毫米。
