Randall H W, Monticello T M, Morgan K T
Department of Experimental Pathology and Toxicology, Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
Stain Technol. 1988 Nov;63(6):355-62. doi: 10.3109/10520298809107611.
The nasal region is important for studies in inhalation toxicology but is difficult to prepare for histological examination, especially in species as large as primates. A method for the histologic preparation of undecalcified, complete transverse sections of the nonhuman primate nasal cavity is summarized as follows. After removal of excess soft tissue, mandible and calvaria, the head is fixed in 10% neutral buffered formalin. The nasal region is transversely sectioned into serial 3-mm-thick blocks from the nares to the posterior aspect of the soft palate using a low speed saw with a water-cooled diamond-coated blade. The blocks are embedded in a mixture of glycol and methyl methacrylates, with polyethylene glycol-1500 and dibutylphthalate as plasticizers. The plastic blocks average 5.0 x 5.0 x 1.5 cm; 2-4 microns sections are cut on an automated sliding microtome. In spite of the size of the blocks, this technique yields complete transverse sections of the nasal cavities with excellent morphologic detail. The sections are amenable to a wide range of staining procedures. The procedure lends itself to autoradiographic studies. The embedding mixture is ideally suited for studies of undecalcified bone and teeth.
鼻腔区域对于吸入毒理学研究很重要，但难以制备用于组织学检查，尤其是对于像灵长类动物这么大的物种。以下总结了一种用于制备非人灵长类动物鼻腔未脱钙完整横切片的组织学方法。去除多余的软组织、下颌骨和颅盖骨后，将头部固定在10%中性缓冲福尔马林中。使用带有水冷金刚石涂层刀片的低速锯，将鼻腔区域从鼻孔到软腭后部横向切成连续的3毫米厚块。将这些块包埋在乙二醇和甲基丙烯酸甲酯的混合物中，以聚乙二醇-1500和邻苯二甲酸二丁酯作为增塑剂。塑料块平均尺寸为5.0×5.0×1.5厘米；在自动滑动切片机上切成2-4微米厚的切片。尽管块体尺寸较大，但该技术能产生具有出色形态细节的鼻腔完整横切片。这些切片适用于广泛的染色程序。该程序适用于放射自显影研究。包埋混合物非常适合未脱钙骨和牙齿的研究。
