Semba R
Stain Technol. 1979 Sep;54(5):251-5. doi: 10.3109/10520297909110680.
Procedures for obtaining sections 1 micrometer thick on a conventional rotary microtome are described. Hydrophilic resin blocks with adequate hardness and elasticity for semithin sectioning are made by addition of divinylbenzene and methylmethacrylate to a commercial embedding kit. The blocks are pinched between two simple adapters and mounted in the specimen holder of a microtome. A glass knife of the Ralph type with an effective blade length of 25 mm is made from a glass slide and attached to a metal bar with paraffin. The low cost assembly is set in the steel knife holder of a conventional rotary microtome. Sections 1 micron in thickness can be cut from the resin embedded blocks. Staining with the usual staining solutions may be weak due to the thinness of the sections, but the fine resolution and low distortion achieved are compensating gains.
本文描述了在传统旋转切片机上获取1微米厚切片的步骤。通过向商用包埋试剂盒中添加二乙烯基苯和甲基丙烯酸甲酯，制成具有足够硬度和弹性以进行半薄切片的亲水性树脂块。将这些树脂块夹在两个简单的适配器之间，并安装在切片机的标本夹中。用载玻片制作有效刀片长度为25毫米的拉尔夫型玻璃刀，并用石蜡将其固定在金属棒上。将这个低成本组件安装在传统旋转切片机的钢刀架中。可以从树脂包埋块上切出1微米厚的切片。由于切片很薄，用常规染色液染色可能效果不佳，但所获得的高分辨率和低失真足以弥补这一不足。
