基于新型功能化导电聚合物的DNA杂交无标记检测

Label-free detection of DNA hybridization based on a novel functionalized conducting polymer.

作者信息

Peng Hui, Soeller Christian, Vigar Nicholas A, Caprio Vittorio, Travas-Sejdic Jadranka

机构信息

Polymer Electronics Research Centre, The University of Auckland, Private Bag 92019, Auckland, New Zealand.

出版信息

Biosens Bioelectron. 2007 Apr 15;22(9-10):1868-73. doi: 10.1016/j.bios.2006.07.010. Epub 2006 Aug 17.

DOI:10.1016/j.bios.2006.07.010

PMID:16919441

Abstract

A new functionalized pyrrole monomer, 3-pyrrolylacrylic acid (PAA) was synthesized. It was used to prepare a copolymer with pyrrole, poly(Py-co-PAA), which was investigated by reflective FT-IR, UV-vis spectroscopy and cyclic voltammetry. A label-free DNA sensor was prepared based on a poly(Py-co-PAA) film. Hybridization with complementary and non-complementary DNA targets was studied by electrochemical impedance spectroscopy. Results show a significant increase in the charge-transfer resistance upon addition of complementary target. The impedance spectra were analyzed by using a modified Randles and Ershler equivalent circuit model. The change in charge-transfer resistance that was used as an index of sensor response was found to be linear with logarithmic target concentration in the range of 2 x 10(-9) to 2 x 10(-7)M. The detection limit was 0.98 nM.

摘要

合成了一种新型功能化吡咯单体3-吡咯丙烯酸（PAA）。用它与吡咯制备了共聚物聚（吡咯-共-PAA），并通过反射傅里叶变换红外光谱、紫外可见光谱和循环伏安法对其进行了研究。基于聚（吡咯-共-PAA）膜制备了一种无标记DNA传感器。通过电化学阻抗谱研究了与互补和非互补DNA靶标的杂交情况。结果表明，加入互补靶标后电荷转移电阻显著增加。使用改进的兰德尔和埃尔施勒等效电路模型对阻抗谱进行了分析。发现用作传感器响应指标的电荷转移电阻变化与2×10⁻⁹至2×10⁻⁷M范围内的对数靶标浓度呈线性关系。检测限为0.98 nM。