Finger T E, St Jeor V L, Kinnamon J C, Silver W L
Rocky Mountain Taste and Smell Center, University of Colorado Health Sciences Center, Denver 80262.
J Comp Neurol. 1990 Apr 8;294(2):293-305. doi: 10.1002/cne.902940212.
The respiratory and olfactory mucosae of rats and mice were examined at ultrastructural levels for the presence of intraepithelial nerve endings. Immunocytochemical studies utilizing antisera directed against substance P and calcitonin gene-related peptide (CGRP) revealed numerous intraepithelial peptide-immunoreactive fibers near the basal region of the epithelium. Occasional transepithelial fibers were observed to extend outward to nearly reach the epithelial surface. In no cases, however, did the transepithelial fibers reach the surface, but instead, stopped at the line of tight junctions approximately 1 micron from the surface. No specialized contacts between the nerve fibers and the epithelial cells were observed. The transepithelial fibers provide a possible anatomical substrate for the sensitivity of the trigeminal nerve to many air-borne chemical stimuli. That potential chemical stimuli must traverse the tight-junctional barrier may explain why lipid solubility is related to effectiveness for trigeminal stimuli.
在超微结构水平上检查大鼠和小鼠的呼吸道及嗅觉黏膜，以确定上皮内神经末梢的存在。利用针对P物质和降钙素基因相关肽（CGRP）的抗血清进行的免疫细胞化学研究显示，上皮基底区域附近有大量上皮内肽免疫反应性纤维。偶尔观察到跨上皮纤维向外延伸，几乎到达上皮表面。然而，在任何情况下，跨上皮纤维都未到达表面，而是在距表面约1微米的紧密连接线上停止。未观察到神经纤维与上皮细胞之间有特殊接触。跨上皮纤维为三叉神经对许多空气传播化学刺激的敏感性提供了一种可能的解剖学基础。潜在的化学刺激必须穿过紧密连接屏障，这可能解释了为什么脂溶性与三叉神经刺激的有效性有关。
