Matsumoto K, Yamada K, Hayakawa T, Sakaguchi T, Mogami H
Department of Neurosurgery, Osaka University Medical School, Japan.
Neurol Res. 1990 Mar;12(1):45-8. doi: 10.1080/01616412.1990.11739912.
Ribonucleic acid (RNA) synthesis was investigated in gerbils subjected to 15 min transient hindbrain ischaemia using [2-14C]uridine autoradiography. Distribution of synthesized RNA in the subcellular fraction of the tissue was detected by differential centrifugation and density gradient separation using Whittaker's method. In [2-14C]uridine autoradiography, uptake of the tracer into the RNA fraction was not reduced after transient ischaemia. Distributional analysis of [2-14C]uridine in the subcellular fractions revealed that tracer activity in the P3 (microsomes) fraction decreased in the ischaemic regions and tended to decrease in the P4 (ribosomes) fraction, although not significantly. Tracer activity in the P1 (nuclei and cell debris) and P2 (mitochondria, myelin and nerve ending particles) fractions did not decrease. These results indicate that RNA synthesis in the nuclei is not inhibited by ischaemia, but RNA processing is disturbed by the level of the transport. Modification of RNA synthesis and processing by transient ischaemia may influence protein synthesis.
采用[2-¹⁴C]尿苷放射自显影技术，对经历15分钟短暂后脑缺血的沙鼠的核糖核酸（RNA）合成进行了研究。使用惠特克方法，通过差速离心和密度梯度分离检测合成RNA在组织亚细胞组分中的分布。在[2-¹⁴C]尿苷放射自显影中，短暂缺血后示踪剂进入RNA组分的摄取并未减少。[2-¹⁴C]尿苷在亚细胞组分中的分布分析显示，缺血区域中P3（微粒体）组分的示踪剂活性降低，P4（核糖体）组分中的示踪剂活性有降低趋势，但不显著。P1（细胞核和细胞碎片）和P2（线粒体、髓鞘和神经末梢颗粒）组分中的示踪剂活性未降低。这些结果表明，缺血并不抑制细胞核中的RNA合成，但RNA加工受到转运水平的干扰。短暂缺血对RNA合成和加工的改变可能会影响蛋白质合成。
