Matsumoto K, Yamada K, Hayakawa T, Yoshimine T, Sakaguchi T, Ushio Y, Akagi K, Mogami H
Department of Neurosurgery, Osaka University Medical School, Japan.
Neurol Res. 1988 Mar;10(1):25-31. doi: 10.1080/01616412.1988.11739811.
Regional protein synthesis was estimated autoradiographically in a model of transient hindbrain ischaemia of gerbils. In studies of 5 min ischaemia followed by 5 min recirculation, incorporation of [14C]valine into the TCA-insoluble protein fraction was not affected. In studies of 15 min ischaemia followed by 5 min recirculation, incorporation of the tracer into the protein fraction was severely depressed in the ischaemic lesion of the brain stem and cerebellum. However, the granular layer of the cerebellar cortex had partially preserved protein synthesis. When recirculation time was extended to 2 h after 30 min ischaemia, protein synthesis of the cerebellar cortex almost recovered to the full level of the control. However, in the pontine grey matter, inferior colliculus and vestibular nucleus, a significant reduction in protein synthesis persisted. These results indicate that protein synthesis in the pontine grey matter, inferior colliculus and vestibular nucleus are selectively inhibited by ischaemia. Further its recovery after recirculation is slow. The cerebellar cortex is less vulnerable to ischaemia, and recovery is relatively fast. The regional heterogeneity of protein synthesis is neither due to the degree of ischaemia in this model nor the extent of postischaemic hypoperfusion. Factors that influence protein synthesis in this ischaemic model are discussed.
在沙土鼠短暂性后脑缺血模型中，通过放射自显影法估计局部蛋白质合成。在5分钟缺血后再灌注5分钟的研究中，[14C]缬氨酸掺入三氯乙酸不溶性蛋白质部分不受影响。在15分钟缺血后再灌注5分钟的研究中，示踪剂掺入蛋白质部分在脑干和小脑的缺血性病变中严重受抑。然而，小脑皮质颗粒层的蛋白质合成部分保留。当缺血30分钟后再灌注时间延长至2小时，小脑皮质的蛋白质合成几乎恢复到对照的完整水平。然而，在脑桥灰质、下丘和前庭核，蛋白质合成持续显著减少。这些结果表明，脑桥灰质、下丘和前庭核的蛋白质合成被缺血选择性抑制。此外，再灌注后其恢复缓慢。小脑皮质对缺血较不敏感，恢复相对较快。蛋白质合成的区域异质性既不是由于该模型中的缺血程度，也不是由于缺血后灌注不足的程度。讨论了影响该缺血模型中蛋白质合成的因素。
