Nieto A, Mira E, Castaño J G
Departamento de Bioquímica, Facultad de Medicina de la UAM, Madrid, Spain.
Biochem J. 1990 Apr 15;267(2):317-23. doi: 10.1042/bj2670317.
The mRNA encoding for rat protein disulphide-isomerase (PDI) increases 3-fold in the liver of diabetic rats and is accompanied by similar changes at the protein level. Long treatment (for 3 days) of diabetic rats with insulin reverses this effect of diabetes both at the mRNA and protein levels. The higher expression of rat PDI mRNA in diabetes is due to an increase in the transcriptional rate of the gene, and insulin treatment of diabetic animals produces within 30 min a decrease in the level of transcription of PDI gene, as judged by nuclear run-on transcription experiments performed in vivo. These results clearly show a role for insulin in the regulation of transcription of the gene encoding this multifunctional protein in rat liver.
编码大鼠蛋白质二硫键异构酶（PDI）的mRNA在糖尿病大鼠肝脏中增加了3倍，并且在蛋白质水平上也有类似变化。用胰岛素对糖尿病大鼠进行长时间（3天）治疗可在mRNA和蛋白质水平上逆转糖尿病的这种影响。糖尿病时大鼠PDI mRNA的较高表达是由于该基因转录速率增加，通过体内进行的核转录实验判断，用胰岛素治疗糖尿病动物在30分钟内可使PDI基因转录水平降低。这些结果清楚地表明胰岛素在调节大鼠肝脏中编码这种多功能蛋白质的基因转录中发挥作用。
