从鸡细胞中纯化色氨酸转移RNA及其与劳氏肉瘤病毒“斑点1”RNA的同一性
Purification of tryptophan transfer RNA from chick cells and its identity with "spot 1" RNA of Rous sarcoma virus.
作者信息
Waters L C, Yang W K, Mullin B C, Nichols J L
出版信息
J Biol Chem. 1975 Aug 25;250(16):6627-9.
PMID:169254
Abstract
Tryptophan transfer RNA from chick cells chromatographs differently in the reversed-phase column chromatographic system 5 depending upon whether it is aminoacylated or not. This property was utilized to prepare pure tryptophan tRNA. Oligonucleotide fingerprints of tryptophan tRNA purified in this manner are identical with those reported for "spot 1" RNA isolated from Rous sarcoma virus.
摘要
来自鸡细胞的色氨酸转运RNA在反相柱色谱系统5中的色谱行为因其是否被氨酰化而有所不同。利用这一特性制备了纯的色氨酸tRNA。以这种方式纯化的色氨酸tRNA的寡核苷酸指纹图谱与从劳氏肉瘤病毒中分离出的“斑点1”RNA的指纹图谱相同。
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