Kukkonen Kaarina, Nieminen Tea, Poussa Tuija, Savilahti Erkki, Kuitunen Mikael
Skin and Allergy Hospital, University of Helsinki, Helsinki, Finland.
Pediatr Allergy Immunol. 2006 Sep;17(6):416-21. doi: 10.1111/j.1399-3038.2006.00420.x.
Probiotics are immunomodulatory and may thus affect vaccine antibody responses. With the accumulating evidence of their health-promoting effects, probiotics are increasingly administered in allergy-prone infants. Therefore, we studied the effect of probiotics on antibody responses to diphtheria, tetanus and Haemophilus influenzae type b (Hib) vaccines in 6-month-old infants participating in a randomized placebo-controlled double-blind allergy-prevention trial. Mothers of unborn children at increased risk for atopy used a combination of four probiotic strains, or a placebo, for 4 wk before delivery. During 6 months from birth, their infants received the same probiotics and galacto-oligosaccharides, or a placebo. The infants were immunized with a DTwP (diphtheria, tetanus and whole cell pertussis) at ages 3, 4, and 5 months, and with a Hib polysaccharide conjugate at 4 months. Serum diphtheria, tetanus, and Hib IgG antibodies were measured at 6 months. In the probiotic group, protective Hib antibody concentrations (>/=1 microg/ml) occurred more frequently, 16 of 32 (50%) vs. six of 29 (21%) (p = 0.020), and the geometric mean (inter-quartile range) Hib IgG concentration tended to be higher 0.75 (0.15-2.71) microg/ml than in the placebo group 0.40 (0.15-0.92) microg/ml (p = 0.064). In these respective groups, diphtheria, 0.38 (0.14-0.78) vs. 0.47 (0.19-1.40) IU/ml (p = 0.449), and tetanus, 1.01(0.47-1.49) vs. 0.81 (0.56-1.39) IU/ml (p = 0.310), IgG titers were comparable. In conclusion, in allergy-prone infants probiotics seem not to impair antibody responses to diphtheria, tetanus, or Hib, but may improve response to Hib immunization.
益生菌具有免疫调节作用，因此可能会影响疫苗抗体反应。随着其促进健康作用的证据不断积累，益生菌越来越多地应用于易患过敏症的婴儿。因此，我们在一项随机、安慰剂对照、双盲的过敏预防试验中，研究了益生菌对6个月大婴儿白喉、破伤风和b型流感嗜血杆菌（Hib）疫苗抗体反应的影响。患特应性疾病风险增加的未出生婴儿的母亲在分娩前4周使用四种益生菌菌株的组合或安慰剂。从出生后的6个月内，她们的婴儿接受相同的益生菌和低聚半乳糖或安慰剂。婴儿在3、4和5个月大时接种白喉、破伤风和全细胞百日咳（DTwP）疫苗，并在4个月大时接种Hib多糖结合疫苗。在6个月时测量血清白喉、破伤风和Hib IgG抗体。在益生菌组中，保护性Hib抗体浓度（≥1微克/毫升）出现的频率更高，32例中有16例（50%），而安慰剂组29例中有6例（21%）（p = 0.020），且几何平均（四分位间距）Hib IgG浓度倾向于高于安慰剂组，分别为0.75（0.15 - 2.71）微克/毫升和0.40（0.15 - 0.92）微克/毫升（p = 0.064）。在这些相应的组中，白喉抗体滴度分别为0.38（0.14 - 0.78）国际单位/毫升和0.47（0.19 - 1.40）国际单位/毫升（p = 0.449），破伤风抗体滴度分别为1.01（0.47 - 1.49）国际单位/毫升和0.81（0.56 - 1.39）国际单位/毫升（p = 0.310），二者相当。总之，在易患过敏症的婴儿中，益生菌似乎不会损害对白喉、破伤风或Hib的抗体反应，但可能会改善对Hib免疫接种的反应。
