Williams M C, Wissig S L
J Cell Biol. 1975 Sep;66(3):531-55. doi: 10.1083/jcb.66.3.531.
The main objective of this study was to determine the pathways by which horseradish peroxidase (HRP) can cross the endothelium of muscle capillaries. Specimens of mouse diaphragm were fixed for cytochemical analysis at various intervals after intervenous injection of 0.5 mg HRP, at 4 min after intervenous injection of varied amounts of HRP, and at 4 min after intervenous injections in various volumes of isotonic NaCl. Our findings indicate that endothelial junctions serve as a barrier which may allow passage of very limited amounts of HRP. They also suggest that endothelial vesicles transfer HRP from the capillary lumen to the pericapillary interstitium as well as in the reverse direction. Increasing the volume of solution injected to approximately 30% of total blood volume did not increase the amount of HRP that left the capillary lumen. Our results with HRP do not provide clearcut evidence that endothelial junctions are the site of the small pore.
本研究的主要目的是确定辣根过氧化物酶（HRP）穿过肌肉毛细血管内皮的途径。在静脉注射0.5毫克HRP后的不同时间间隔、静脉注射不同剂量HRP后4分钟以及静脉注射不同体积等渗氯化钠溶液后4分钟，对小鼠膈肌标本进行固定以进行细胞化学分析。我们的研究结果表明，内皮连接作为一种屏障，可能仅允许极少量的HRP通过。研究结果还表明，内皮小泡可将HRP从毛细血管腔转运至毛细血管周围间质，反之亦然。将注射溶液的体积增加至约总血容量的30%，并未增加离开毛细血管腔的HRP量。我们使用HRP的研究结果并未提供明确证据表明内皮连接是小孔的所在位置。
