Ozaki S, Kalant N
J Lab Clin Med. 1977 Oct;90(4):686-99.
A method is described for the measurement of insulin in serum by RRA with normal human sRRA. The sensitivity of the assay is similar to that of a commonly used immunoassay. There is no detectable interference from somatomedin or NSILA, whereas proinsulin has only about 5% of the activity of insulin in the assay. There is a high degree of correlation between the results of immunoassay and the RRA over the physiological range of serum insulin. However, the greater ability to differentiate between insulin and proinsulin, together with greater ability of receptor binding to exclude altered (biologically inactive) insulin, present advantages of this assay over the RIA, which may be significant in abnormal situations.
本文描述了一种使用正常人可溶性受体放射分析法（sRRA）测定血清中胰岛素的方法。该测定法的灵敏度与常用免疫测定法相似。生长调节素或非抑制胰岛素样活性（NSILA）无明显干扰，而胰岛素原在该测定法中的活性仅约为胰岛素的5%。在血清胰岛素的生理范围内，免疫测定法和受体放射分析法的结果之间具有高度相关性。然而，该测定法在区分胰岛素和胰岛素原方面能力更强，且受体结合能更好地排除改变的（生物无活性的）胰岛素，这使其相对于放射免疫分析法（RIA）具有优势，在异常情况下可能具有重要意义。
