Cullen Bryan R
Center for Virology, and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Nat Methods. 2006 Sep;3(9):677-81. doi: 10.1038/nmeth913.
RNA interference (RNAi) provides a powerful technique for the derivation and analysis of loss-of-function phenotypes in vertebrate cells, where alternative approaches are either arduous or frequently ineffective. RNAi, however, is not always totally specific, and confirming the specificity, and hence the validity, of data obtained using RNAi therefore forms an essential component of experiments that rely on this technique. Here I briefly review the potential pitfalls associated with RNAi, and then suggest experimental approaches that can be used to maximize the specificity of RNAi or to confirm that the data obtained are indeed valid.
RNA干扰（RNAi）为脊椎动物细胞中功能缺失表型的推导和分析提供了一种强大的技术，而其他替代方法要么艰巨，要么常常无效。然而，RNAi并不总是完全特异的，因此，确认使用RNAi获得的数据的特异性以及有效性，是依赖该技术的实验的重要组成部分。在此，我简要回顾与RNAi相关的潜在陷阱，然后提出一些实验方法，可用于最大化RNAi的特异性或确认所获得的数据确实有效。
