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蛋白激酶C活性在白细胞介素-1（IL-1）诱导白细胞介素-2（IL-2）基因表达中起作用，但在IL-1信号转导中不起作用。

A role for protein kinase C activity in interleukin-1 (IL-1) induction of IL-2 gene expression but not in IL-1 signal transduction.

作者信息

Macchia G, Baldari C T, Massone A, Telford J L

机构信息

Sclavo Research Centre, Siena, Italy.

出版信息

Mol Cell Biol. 1990 Jun;10(6):2731-7. doi: 10.1128/mcb.10.6.2731-2737.1990.

DOI:10.1128/mcb.10.6.2731-2737.1990
PMID:1692959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360633/
Abstract

Interleukin-1 (IL-1) is known to synergize with phorbol esters in the induction of interleukin-2 (IL-2) expression in T-lymphoid leukemia cells and proliferation of mouse thymocytes. We used a plasmid construct containing the bacterial gene for chloramphenicol acetyltransferase under the control of the human IL-2 promoter to study the nature of this synergism in the murine thymoma cell line EL4. Although IL-1 induction of the IL-2 promoter in these cells required costimulus with phorbol myristate acetate, the signal induced by IL-1 was qualitatively different. We provide evidence to support the hypothesis that the phorbol ester signal is mediated by protein kinase C, and we show that the IL-1 signal is not. That IL-1 and phorbol myristate acetate represent different stimuli was shown by their response to protein kinase C inhibitors, capacity to synergize with increased intracellular free calcium, and requirement for protein synthesis. In addition we show that pretreatment with IL-1 can prime EL4 cells to subsequent activation by concentrations of phorbol esters not normally sufficient to induce IL-2 expression. Pretreated cells remained primed for at least 40 h after removal of the IL-1. Neither phorbol myristate acetate nor a calcium ionophore was capable of preactivating EL4 cells.

摘要

白细胞介素-1（IL-1）已知可与佛波酯协同作用，诱导T淋巴细胞白血病细胞中白细胞介素-2（IL-2）的表达以及小鼠胸腺细胞的增殖。我们使用了一种质粒构建体，其包含在人IL-2启动子控制下的氯霉素乙酰转移酶细菌基因，以研究鼠胸腺瘤细胞系EL4中这种协同作用的性质。尽管在这些细胞中IL-1对IL-2启动子的诱导需要与佛波醇肉豆蔻酸酯共同刺激，但IL-1诱导的信号在性质上有所不同。我们提供证据支持佛波酯信号由蛋白激酶C介导的假说，并且表明IL-1信号并非如此。IL-1和佛波醇肉豆蔻酸酯代表不同刺激这一点，通过它们对蛋白激酶C抑制剂的反应、与细胞内游离钙增加协同作用的能力以及对蛋白质合成的需求得以体现。此外，我们表明用IL-1预处理可使EL4细胞对随后通常不足以诱导IL-2表达的佛波酯浓度的激活产生预致敏。去除IL-1后，预处理的细胞至少40小时内仍保持预致敏状态。佛波醇肉豆蔻酸酯和钙离子载体均无法预激活EL4细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/78ea6d554179/molcellb00042-0305-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/a4b48e711e38/molcellb00042-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/ff87338a6394/molcellb00042-0303-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/37fec6b1e176/molcellb00042-0303-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/4b1358a229ee/molcellb00042-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/f4c1d31c0071/molcellb00042-0304-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/30244de9e8e8/molcellb00042-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/d1644a3ff33e/molcellb00042-0305-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/b3f703f9d795/molcellb00042-0305-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/78ea6d554179/molcellb00042-0305-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/a4b48e711e38/molcellb00042-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/ff87338a6394/molcellb00042-0303-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/37fec6b1e176/molcellb00042-0303-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/4b1358a229ee/molcellb00042-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/f4c1d31c0071/molcellb00042-0304-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/30244de9e8e8/molcellb00042-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/d1644a3ff33e/molcellb00042-0305-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/b3f703f9d795/molcellb00042-0305-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80ed/360633/78ea6d554179/molcellb00042-0305-d.jpg

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本文引用的文献

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Two distinct factors are required for induction of T-cell growth.诱导T细胞生长需要两个不同的因素。
Nature. 1980 Feb 14;283(5748):664-6. doi: 10.1038/283664a0.
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
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pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
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Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C.异喹啉磺酰胺，新型强效环核苷酸依赖性蛋白激酶和蛋白激酶C抑制剂。
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The role of protein kinase C in cell surface signal transduction and tumour promotion.蛋白激酶C在细胞表面信号转导及肿瘤促进中的作用。
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