Fujita T, Takaoka C, Matsui H, Taniguchi T
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7437-41. doi: 10.1073/pnas.80.24.7437.
We have cloned two species of EcoRI-cleaved DNA segments that together cover the entire sequence for the human interleukin 2 gene and have determined the nucleotide sequence of the gene and its flanking regions. The gene contains three introns and the exon sequences can be aligned with the previously reported cDNA sequence almost perfectly except for a few nucleotides in the 3' nontranslated region. The promoter region contains a prototype "TATA" sequence as well as a notable palindromic sequence. Particularly interesting is the presence of sequences in this region that are homologous to the promoter region of the human interferon-gamma gene. In addition, a sequence that closely resembles the core sequence for the viral enhancer elements has been found in the second intron. Such sequences may play a role in the expression of the interleukin 2 gene in lectin- or antigen-stimulated T lymphocytes.
我们克隆了两种经EcoRI切割的DNA片段，它们共同覆盖了人类白细胞介素2基因的完整序列，并确定了该基因及其侧翼区域的核苷酸序列。该基因包含三个内含子，外显子序列与先前报道的cDNA序列几乎完美匹配，除了3'非翻译区的少数核苷酸。启动子区域包含一个典型的“TATA”序列以及一个显著的回文序列。特别有趣的是，该区域存在与人类干扰素-γ基因启动子区域同源的序列。此外，在第二个内含子中发现了一个与病毒增强子元件核心序列非常相似的序列。这些序列可能在凝集素或抗原刺激的T淋巴细胞中白细胞介素2基因的表达中发挥作用。
