Pavlenko A F, Kurika A V, Chicalovets I V
Pacific Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Vladivostok.
Tumour Biol. 1990;11(3):137-44. doi: 10.1159/000217648.
Oncoprecipitins isolated from a number of marine invertebrates have been shown to be glycoproteins possessing a higher specificity to carcinoembryonic antigen (CEA)--similar to the interaction of antigen-antibody. The oncoprecipitin-CEA interaction was found to be substantially more specific than that of carbohydrate-containing polymers with lectins. Two oncoprecipitins, crustacin and cyprein, isolated from Pagurus prideauxii and Cyprea caputserpentis, respectively, have been characterized. The binding reaction of CEA with crustacin and cyprein failed to be inhibited with glycoproteins having sugar chain patterns closely related to those of CEA. Inhibition of the oncoprecipitin-CEA interaction with sugars was shown to be nonspecific and was observed only at sufficiently high concentrations of sugars used as inhibitors. Immunoenzymic assay of CEA content in normal and tumor tissues using CEA crustacin and CEA antiserum against CEA test systems revealed highly consistent results for both systems.
从多种海洋无脊椎动物中分离出的癌沉淀素已被证明是糖蛋白，对癌胚抗原（CEA）具有更高的特异性——类似于抗原-抗体的相互作用。发现癌沉淀素与CEA的相互作用比含碳水化合物的聚合物与凝集素的相互作用特异性更强。分别从光掌寄居蟹和蛇首眼球贝中分离出的两种癌沉淀素，即甲壳蛋白和螺血蓝蛋白，已得到鉴定。CEA与甲壳蛋白和螺血蓝蛋白的结合反应不能被糖链模式与CEA密切相关的糖蛋白所抑制。癌沉淀素与CEA相互作用的糖抑制作用是非特异性的，仅在用作抑制剂的糖浓度足够高时才会观察到。使用CEA甲壳蛋白和针对CEA检测系统的CEA抗血清对正常组织和肿瘤组织中的CEA含量进行免疫酶测定，结果显示两个系统的结果高度一致。
