Pavlenko A F, Kurika A V, Chikalovets I V, Belogortseva N I, Ovodov Iu S
Bioorg Khim. 1988 Jan;14(1):58-64.
Crustacin from Pagurus prideauxii and cyprein from Cyprea caputserpentis have been shown to be glycoproteins with molecular masses 36 +/- 1 and 44 +/- 1.4 kDa, containing 3 and 18% of carbohydrates, respectively. Their amino acid and monosaccharide composition have been determined. Crustacin or cyprein interact with CEA more specifically than carbohydrate-containing polymers with lectins. Carbohydrates unspecifically inhibit this interaction at rather high concentrations. Association constants of CEA-crustacin and CEA-cyprein complexes are 0.6.10(8) M-1, respectively. Besides, ELISA showed that antibodies against CEA bind antibodies to crustacin and cyprein.
已证明来自骄傲寄居蟹的甲壳蛋白和来自蛇首宝螺的宝螺蛋白是糖蛋白，分子量分别为36±1 kDa和44±1.4 kDa，分别含有3%和18%的碳水化合物。已测定它们的氨基酸和单糖组成。甲壳蛋白或宝螺蛋白与癌胚抗原（CEA）的相互作用比含碳水化合物的聚合物与凝集素的相互作用更具特异性。碳水化合物在相当高的浓度下非特异性地抑制这种相互作用。CEA-甲壳蛋白和CEA-宝螺蛋白复合物的缔合常数分别为0.6×10⁸ M⁻¹。此外，酶联免疫吸附测定（ELISA）表明，抗CEA抗体能结合抗甲壳蛋白和宝螺蛋白的抗体。
