Dolin R, Smith H A
J Infect Dis. 1975 Sep;132(3):287-95. doi: 10.1093/infdis/132.3.287.
In vitro antiviral activities of two potentially clinically useful antiviral compounds, adenine arabinoside and iododeoxyuridine, were examined in human fetal intestinal and tracheal organ cultures infected with Herpesvirus hominis (types 1 and 2) or vaccinia virus. The two compounds were similarly active against the viruses in organ culture, and minimal inhibitory concentrations could be determined by titration of organ culture fluid harvests into tissue culture or directly in the organ cultures themselves. Minimal inhibitory concentrations were consistently lower in tracheal than in intestinal organ cultures and were consistently higher for H. hominis type 2 than for H. hominis type 1. Thus the organ cultures are promising systems in which to evaluate antiviral activity against those agents that replicate in vitro only in organ culture, and they may have particular application to the study of herpetic tracheitis and esophagitis.
在用人胎儿肠道和气管器官培养物感染人疱疹病毒（1型和2型）或痘苗病毒的实验中，检测了两种具有潜在临床应用价值的抗病毒化合物——阿糖腺苷和碘脱氧尿苷的体外抗病毒活性。这两种化合物在器官培养物中对病毒的活性相似，通过将器官培养液收获物滴定到组织培养物中或直接在器官培养物本身中进行滴定，可以确定最低抑菌浓度。气管器官培养物中的最低抑菌浓度始终低于肠道器官培养物，人疱疹病毒2型的最低抑菌浓度始终高于人疱疹病毒1型。因此，器官培养是评估针对仅在器官培养物中体外复制的病原体的抗病毒活性的有前景的系统，并且它们可能在疱疹性气管炎和食管炎的研究中具有特殊应用。
