Department of Ophthalmology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Department of Anatomy, Wannan Medical College, Wuhu, China.
Transl Vis Sci Technol. 2021 Nov 1;10(13):30. doi: 10.1167/tvst.10.13.30.
To investigate the effects of indocyanine green (ICG) solution on the viability and cytolysis of human lens epithelial cells ex vivo.
A total of 200 pieces of anterior capsules were obtained during cataract surgery, and 110 pieces of the anterior capsules were randomly divided into five groups and treated by immersion in different concentrations of ICG solution. The remaining 90 anterior capsules were also divided into five groups and treated with a combination of drug immersion and washing in balanced salt solution. Electron microscopy and trypan blue and eosin stains were used to analyze the cells. Percentage of dead, shedding, or living lens epithelial cells was estimated and used to demonstrate effects of the ICG on viability and cytolysis.
Compared with the control group, the percentage of dead and shedding lens epithelial cells increased while the percentage of living lens epithelial cells decreased in all the immersion groups. In the washing groups, the percentage of the living lens epithelial cells was 63.42% ± 2.49%, 54.04% ± 1.84%, 43.51% ± 2.63%, 29.21% ± 2.40%, and 15.73% ± 1.61% for the five groups and reflected a concentration-effect relationship. Electron microscopy showed that the higher the concentration of the ICG solution, the more severe the destruction of the lens epithelial cell structure.
ICG could reduce the viability of the lens epithelial cells and promote cell cytolysis.
Our study showed that ICG could directly reduce the viability of the lens epithelial cells in a concentration-dependent fashion, which can theoretically reduce the incidence of posterior capsule opacification.
研究吲哚菁绿（ICG）溶液对人晶状体上皮细胞活力和细胞溶解的影响。
在白内障手术中获得 200 片前囊，随机将 110 片前囊分为五组，分别浸泡在不同浓度的 ICG 溶液中。其余 90 片前囊也分为五组，采用药物浸泡联合平衡盐溶液冲洗处理。利用电子显微镜和台盼蓝和伊红染色分析细胞。估计死亡、脱落或存活的晶状体上皮细胞的比例，以证明 ICG 对活力和细胞溶解的影响。
与对照组相比，所有浸泡组的死亡和脱落晶状体上皮细胞比例增加，而存活晶状体上皮细胞比例降低。在冲洗组中，存活的晶状体上皮细胞比例分别为 63.42%±2.49%、54.04%±1.84%、43.51%±2.63%、29.21%±2.40%和 15.73%±1.61%，呈浓度依赖性。电子显微镜显示，ICG 溶液浓度越高，晶状体上皮细胞结构破坏越严重。
ICG 可降低晶状体上皮细胞的活力并促进细胞溶解。
我们的研究表明，ICG 可以直接降低晶状体上皮细胞的活力，且呈浓度依赖性，理论上可以降低后发性白内障的发生率。
