Sahbaie Peyman, Modanlou Shohreh, Gharagozlou Parham, Clark J David, Lameh Jelveh, Delorey Timothy M
Molecular Research Institute, Mountain View, California 94043, USA.
Anesth Analg. 2006 Sep;103(3):620-5. doi: 10.1213/01.ane.0000229714.09553.8c.
We assessed a simple, noninvasive method of monitoring transcutaneous partial pressure of CO2 (Ptcco2) in mice to determine whether it would provide an accurate and reproducible method to assess ventilatory depression in mice. To this end, Ptcco2 and Paco2 (partial pressure of arterial CO2) measurements were performed on isoflurane-anesthetized male C57Bl/6 mice breathing differing percentages of CO2 or fentanyl, a known ventilatory depressive drug. All doses of fentanyl produced a sharp increase in Ptcco2 values within 20 min with difference in Ptcco2 values between saline and all fentanyl groups being statistically significant (P < 0.0001). A good correlation between Paco2 and Ptcco2 values was established (r2 = 0.91). A Bland-Altman analysis likewise found that Ptcco2 measurements in the mice reliably and accurately reflected their Paco2 values. Therefore, under controlled conditions, Ptcco2 measurements were found to reliably reflect Paco2 values in mice. Consequently, the Ptcco2 method can be used as a means to rapidly and quantitatively assess the ventilatory depressive properties of a wide spectrum of drugs, under varying conditions in numerous mouse models.
我们评估了一种监测小鼠经皮二氧化碳分压（Ptcco2）的简单、非侵入性方法，以确定它是否能提供一种准确且可重复的方法来评估小鼠的通气抑制。为此，对吸入异氟烷麻醉的雄性C57Bl/6小鼠进行了Ptcco2和动脉血二氧化碳分压（Paco2）测量，这些小鼠呼吸不同百分比的二氧化碳或芬太尼（一种已知的通气抑制药物）。所有剂量的芬太尼在20分钟内均使Ptcco2值急剧升高，生理盐水组与所有芬太尼组之间的Ptcco2值差异具有统计学意义（P < 0.0001）。Paco2与Ptcco2值之间建立了良好的相关性（r2 = 0.91）。Bland-Altman分析同样发现，小鼠的Ptcco2测量值可靠且准确地反映了它们的Paco2值。因此，在受控条件下，发现Ptcco2测量值能可靠地反映小鼠的Paco2值。所以，Ptcco2方法可作为一种手段，在众多小鼠模型的不同条件下，快速且定量地评估多种药物的通气抑制特性。
