Schmitt M P, Steffan A M, Gendrault J L, Jaeck D, Royer C, Schweitzer C, Beyer C, Schmitt C, Aubertin A M, Kirn A
Laboratoire de Virologie, Faculté de Médecine et Unité INSERM U74, Strasbourg, France.
Res Virol. 1990 Mar-Apr;141(2):143-52. doi: 10.1016/0923-2516(90)90016-c.
In primary cultures of Kupffer cells obtained from surgical biopsies of human liver by collagenase perfusion followed by centrifugal elutriation and infected with HIV, the virus multiplied abundantly, as attested by the appearance of a reverse transcriptase activity in the medium. Examined by electron microscopy, the cells were found to contain viral particles with typical features of Lentivirinae. Furthermore, the virus could be revealed by immunofluorescence using an HIV+ patient serum. HIV antibodies also neutralized the infectivity of the Kupffer cell-produced virus. Our results demonstrate that the cells constituting the largest fraction of fixed macrophages in the body may be infected by HIV, thereby suggesting that the Kupffer cells may play a role in the physiopathology of the disease, namely as a reservoir for the virus.
在通过胶原酶灌注、离心淘析从人肝脏手术活检中获得并感染HIV的库普弗细胞原代培养物中，病毒大量繁殖，培养基中出现逆转录酶活性即证明了这一点。通过电子显微镜检查发现，这些细胞含有具有慢病毒科典型特征的病毒颗粒。此外，使用一名HIV阳性患者的血清通过免疫荧光法可检测到该病毒。HIV抗体也可中和库普弗细胞产生的病毒的感染性。我们的结果表明，构成体内固定巨噬细胞最大部分的细胞可能会被HIV感染，从而提示库普弗细胞可能在该疾病的病理生理学中发挥作用，即作为病毒的储存库。
