Shi Shude, Qiu Yingxiong, Li Enxiang, Wu Ling, Fu Chengxin
Laboratory of Systematic and Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou, 310058, PR China.
Biochem Genet. 2006 Jun;44(5-6):198-208. doi: 10.1007/s10528-006-9023-4. Epub 2006 Aug 25.
To examine interspecific relationships and test the hypothesis of hybrid origin within Lycoris species, this study used data from parsimony analyses with nuclear ITS sequences for 19 taxa representing 14 species of Lycoris and two outgroup taxa. The ITS sequences resolved three infrageneric clades. One clade included L. chinensis, L. longituba, L. longituba var. flava, L. anhuiensis, and L. aurea; the second one consisted of L. sprengeri, L. radiata, L. radiata var. radiata, L. radiata var. pumila, L. haywardii, L. rosea, L. sanguinea var. sanguinea, and L. sanguinea var. koreana; and the third included L. caldwellii, L. straminea, L. albiflora, L. flavescens, and two hybrids. The results strongly support the hypothesis that L. straminea originated from hybridization between L. chinensis and L. radiata var. pumila, and the allotriploid L. caldwellii and L. albiflora derived from hybridization between L. chinensis and L. sprengeri. As nucleotide additivity was observed in the artificial hybrids and several presumed hybrids, the likelihood of hybrid origin of Lycoris species is supported.
为了研究石蒜属物种间的关系并验证杂交起源假说，本研究使用了简约分析的数据，这些数据来自代表石蒜属14个物种的19个分类群以及两个外类群分类群的核ITS序列。ITS序列解析出三个亚属分支。一个分支包括中国石蒜、长筒石蒜、长筒石蒜黄花变种、安徽石蒜和忽地笑；第二个分支由换锦花、辐射石蒜、辐射石蒜原变种、矮小石蒜、海沃德石蒜、玫瑰石蒜、血红色石蒜血红色变种和血红色石蒜朝鲜变种组成；第三个分支包括卡氏石蒜、稻草石蒜、白花石蒜、淡黄中国石蒜以及两个杂种。结果有力地支持了稻草石蒜起源于中国石蒜和矮小石蒜杂交的假说，以及异源三倍体卡氏石蒜和白花石蒜源自中国石蒜和换锦花杂交的假说。由于在人工杂种和几个假定杂种中观察到核苷酸加性，石蒜属物种杂交起源的可能性得到了支持。
