用于石蒜（石蒜科）遗传分析的多态性微卫星位点及在其他同属物种中的交叉扩增

Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species.

作者信息

Xuan S Q, Zheng J Y, Wang H, Gao P, Zhou S B, Liu K, Zhu G-P

机构信息

Key Laboratory of Molecular Evolution and Biodiversity, Institute of Molecular Biology and Biotechnology, Anhui Normal University, Wuhu, PR China.

出版信息

Genet Mol Res. 2011 Dec 19;10(4):3141-5. doi: 10.4238/2011.December.19.2.

DOI:10.4238/2011.December.19.2

PMID:22194169

Abstract

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci from 21 L. radiata individuals of a natural population from Yellow Mountain in Anhui Province, China. The number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.238 to 0.952 and from 0.455 to 0.784, respectively. One locus significantly deviated from Hardy-Weinberg equilibrium and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification of these microsatellite loci was characterized in additional five species (L. sprengeri, L. anhuiensis, L. albiflora, L. longituba, and L. chinensis) of Lycoris. The results suggest that these microsatellite markers would contribute to the population genetic studies of L. radiata and other related species.

摘要

石蒜是一种多年生草本植物，长期以来一直用于传统中药，其鳞茎中有两种主要药用成分，即石蒜碱和加兰他敏。然而，尚未针对任何石蒜属物种开发出原始微卫星位点。使用改良的CTAB方案从新鲜鳞茎中提取总基因组DNA。我们从中国安徽省黄山自然种群的21个石蒜个体中分离出10个微卫星位点。等位基因数量从2到9不等。观察到的杂合度和预期杂合度分别为0.238至0.952和0.455至0.784。一个位点显著偏离哈迪-温伯格平衡，并且在成对的位点之间未发现显著的连锁不平衡。在另外五种石蒜属植物（换锦花、安徽石蒜、白花石蒜、长筒石蒜和中国石蒜）中对这些微卫星位点的跨物种扩增进行了表征。结果表明，这些微卫星标记将有助于石蒜及其其他相关物种的种群遗传学研究。