Kalyan Koti, Basu Debdatta, Soundararaghavan Jayanthi
Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry.
Indian J Pathol Microbiol. 2006 Apr;49(2):203-7.
The recent WHO classification of non-Hodgkin's lymphoma is based on the morphology and immunohistochemical expression of the lymphoma cells and to a lesser extent, on the molecular and cytogenetic findings. Fifty-three cases of non-Hodgkin's lymphoma were included in the study. Of these, seven cases were primary extra nodal lymphomas. Twenty two patients had peripheral blood and/or bone marrow involvement. A detailed morphological assessment was done and classified using the International working formulation. The two most common types encountered were diffuse large cell lymphoma and small lymphocytic lymphoma. Immunohistochemistry was done using labeled streptavidin-biotin peroxidase complex with CD3, CD20, CD15, CD30, CD 45 (leukocyte common antigen), Cyclin D1, EMA (epithelial membrane antigen). 38 cases (72%) showed B cell expression and 12 cases (22.5%) showed T cell expression. Three cases did not express either marker. B-cell diffuse large cell lymphoma (26%) was found to be the predominant B cell non-Hodgkin's lymphoma. The commonest T-cell lymphoma was T lymphoblastic lymphoma (67%) followed by peripheral T cell angioimmunoblastic lymphoma (25%). Immunohistochemistry is a useful and necessary diagnostic modality and helps subdivide prognostically different types of non-Hodgkin's lymphoma.
世界卫生组织最近对非霍奇金淋巴瘤的分类基于淋巴瘤细胞的形态学和免疫组化表达，在较小程度上也基于分子和细胞遗传学研究结果。本研究纳入了53例非霍奇金淋巴瘤病例。其中，7例为原发性结外淋巴瘤。22例患者有外周血和/或骨髓受累。进行了详细的形态学评估，并根据国际工作分类法进行分类。最常见的两种类型是弥漫性大细胞淋巴瘤和小淋巴细胞淋巴瘤。免疫组化采用标记链霉亲和素-生物素过氧化物酶复合物检测CD3、CD20、CD15、CD30、CD45（白细胞共同抗原）、细胞周期蛋白D1、EMA（上皮膜抗原）。38例（72%）显示B细胞表达，12例（22.5%）显示T细胞表达。3例未表达任何一种标志物。B细胞弥漫性大细胞淋巴瘤（26%）是主要的B细胞非霍奇金淋巴瘤。最常见的T细胞淋巴瘤是T淋巴母细胞淋巴瘤（67%），其次是外周T细胞血管免疫母细胞性淋巴瘤（25%）。免疫组化是一种有用且必要的诊断方法，有助于对预后不同的非霍奇金淋巴瘤进行细分。
