Ahmad Farook, Christenson Andreas, Bainbridge Martina, Yusof Ahmad Pauzi Mohd, Ab Ghani Sulaiman
Pusat Pengajian Sains Kimia, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Biosens Bioelectron. 2007 Mar 15;22(8):1625-32. doi: 10.1016/j.bios.2006.07.025. Epub 2006 Aug 24.
A new implantable electrocatalytic glucose sensor for subcutaneous glucose monitoring has been fabricated by immobilizing glucose oxidase on a chemically modified carbon fiber. The sensor was inserted subcutaneously on a male spraguely rat without any incision after dipping the microsensor in the rat's serum for 3 days. The so called "stained" microsensor, operated in the amperometric mode with an applied potential of +0.23 V versus Ag|AgCl, was able to directly measure the glucose concentration upon infusion of glucose. The results obtained were encouraging, with the response time was less than 2s and the apparent Michaelis-Menten value at 5.1+/-0.5mM. The "stained" microsensor shows good stability and reproducibility with constant response spanned over 25 days. Most common interferences in glucose analysis were minimized by the outerlayer Nafion. Hematology examinations showed minimal material-tissue interaction. Use of such mechanical devices will allow a more refined understanding towards glucose control in diabetic patients as the implanted microsensor was not effected by biocompatibility failures.
一种用于皮下葡萄糖监测的新型可植入式电催化葡萄糖传感器已通过将葡萄糖氧化酶固定在化学修饰的碳纤维上制成。将微传感器浸入大鼠血清中3天后，在没有任何切口的情况下将该传感器皮下插入一只雄性Sprague-Dawley大鼠体内。所谓的“染色”微传感器，在安培模式下以相对于Ag|AgCl为+0.23 V的外加电位运行，在注入葡萄糖时能够直接测量葡萄糖浓度。获得的结果令人鼓舞，响应时间小于2秒，表观米氏常数为5.1±0.5 mM。“染色”微传感器显示出良好的稳定性和重现性，在25天内具有恒定的响应。外层的Nafion将葡萄糖分析中最常见的干扰降至最低。血液学检查显示材料与组织的相互作用最小。使用这种机械设备将使我们对糖尿病患者的血糖控制有更精确的了解，因为植入的微传感器不受生物相容性故障的影响。
