Jiang Y, Li P, Li S P, Wang Y T, Tu P F
School of Pharmaceutical Sciences, Health Science Center of Peking University, Beijing, China.
J Pharm Biomed Anal. 2007 Jan 4;43(1):341-5. doi: 10.1016/j.jpba.2006.06.030. Epub 2006 Aug 28.
As an alternative of traditional extraction method, pressurized liquid extraction (PLE) was applied for five flavanoids extraction from Lysimachia clethroide. The operational parameters of PLE, such as extraction solvent, temperature, pressure, static extraction time, flush volume and cycles were optimized by univariate approach coupled with central composite design (CCD) in order to obtain the highest extraction efficiency. The optimized result employed 50% acetonitrile aqueous as extraction solvent, 100 degrees C of extraction temperature, 1500 psi of extraction pressure, 25 min of static time, 70% flush volume, and only one cycle to extract the target compounds completely. Finally, the contents of five major flavanoids in L. clethroides from different sources were determined simultaneously by the combination of the presented PLE and HPLC method.
作为传统提取方法的替代方法，采用加压液体萃取（PLE）从珍珠菜中提取5种黄酮类化合物。通过单因素法结合中心复合设计（CCD）对PLE的操作参数，如萃取溶剂、温度、压力、静态萃取时间、冲洗体积和循环次数进行优化，以获得最高的萃取效率。优化结果采用50%乙腈水溶液作为萃取溶剂，萃取温度为100℃，萃取压力为1500 psi，静态时间为25分钟，冲洗体积为70%，仅一个循环即可完全萃取目标化合物。最后，结合所提出的PLE和HPLC方法同时测定了不同来源珍珠菜中5种主要黄酮类化合物的含量。
