Stackebrandt E, Charfreitag O
Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, Kiel, FRG.
J Gen Microbiol. 1990 Jan;136(1):37-43. doi: 10.1099/00221287-136-1-37.
The intra- and intergeneric relationships of the genus Actinomyces were determined by comparing long 16S rRNA sequences, generated by reverse transcriptase. All species formed a phylogenetically coherent cluster in which Actinomyces bovis, A. viscosus, A. naeslundii, A. odontolyticus and A. israelii constituted genetically well defined species. A. israelii DSM 43322 (serotype 2) was not closely related to three other strains of this species (serotype 1) and, as judged from phylogenetic distances, could be accommodated within A. naeslundii, or represent a new species. In contrast to previous findings, members of the genus Actinomyces appear to be related to Bifidobacterium bifidum. Sequence information was used to develop an oligonucleotide probe for the A. israelii serotype 1 strains, which did not react with the serotype 2 strain or with rRNA from strains of eight Actinomyces species.
通过比较逆转录酶产生的长 16S rRNA 序列，确定了放线菌属的属内和属间关系。所有物种形成了一个系统发育连贯的聚类，其中牛放线菌、粘性放线菌、内氏放线菌、溶牙放线菌和衣氏放线菌构成了遗传上定义明确的物种。衣氏放线菌 DSM 43322（血清型 2）与该物种的其他三个菌株（血清型 1）关系不密切，从系统发育距离判断，它可以归入内氏放线菌属，或者代表一个新物种。与先前的发现相反，放线菌属的成员似乎与双歧双歧杆菌有关。序列信息被用于开发一种针对衣氏放线菌血清型 1 菌株的寡核苷酸探针，该探针不与血清型 2 菌株或八种放线菌物种的菌株的 rRNA 发生反应。
