Fernandez L, Doligez A, Lopez G, Thomas M R, Bouquet A, Torregrosa L
UMR BEPC, campus-Agro-M/INRA, Montpellier, CEDEX 01, France.
Genome. 2006 Jul;49(7):721-8. doi: 10.1139/g06-034.
The fleshless berry (flb) mutation of grapevine (Vitis vinifera L. 'Ugni Blanc') impairs the differentiation and division of inner mesocarp cells responsible for flesh in grapevine berries. In order to study the inheritance of the mutation and to map the flb locus, 5 segregating populations were created. Progeny plants were classified as mutant or wild type by scoring for the presence of an ovary phenotype associated with the Flb- phenotype at anthesis. Phenotypic segregation revealed the involvement of a single dominant allele that was heterozygous in the original mutant. Through bulk segregant analysis, microsatellite (simple sequence repeat (SSR)) markers linked to the mutation were identified, and the flb locus was assigned to linkage group 18. The locus position was then refined by analyzing individual progeny and the segregation of SSR markers in the target region with the closest marker 5.6 cM distant from the flb locus. All progeny with the Flb- ovary phenotype differed from the original fleshless berry mutant in that no berries formed after anthesis. Our data suggest that the original mutant plant was a chimera with the mutated allele present in only 1 cell layer (L2 layer) of the ovary and berry.
葡萄（欧亚种葡萄‘白玉霓’）的无果肉浆果（flb）突变会损害葡萄浆果中负责果肉形成的内果皮细胞的分化和分裂。为了研究该突变的遗传方式并定位flb基因座，构建了5个分离群体。通过在花期对与Flb-表型相关的子房表型进行评分，将子代植株分为突变型或野生型。表型分离显示该突变涉及一个单一的显性等位基因，其在原始突变体中为杂合状态。通过混合分组分析法，鉴定出与该突变连锁的微卫星（简单序列重复，SSR）标记，并将flb基因座定位到第18连锁群。随后，通过分析单个子代以及目标区域内SSR标记的分离情况，对基因座位置进行了精细定位，距离flb基因座最近的标记与之相距5.6 cM。所有具有Flb-子房表型的子代与原始无果肉浆果突变体的不同之处在于，花期后不形成浆果。我们的数据表明，原始突变植株是一种嵌合体，突变等位基因仅存在于子房和浆果的1个细胞层（L2层）中。
