Takakura Natsuko, Wakabayashi Hiroyuki, Yamauchi Koji, Takase Mitsunori
Nutritional Science Laboratory, Morinaga Milk Industry Co. Ltd, 5-1-83 Higashihara, Zama, Kanagawa 228-8583, Japan.
Biochem Cell Biol. 2006 Jun;84(3):363-8. doi: 10.1139/o06-056.
Intestinal mucosal immunity plays an important role in mucosal and systemic immune responses. We investigated the influences of orally administered bovine lactoferrin (LF) on cytokine production by intestinal intraepithelial lymphocytes (IEL) and mesenteric lymph-node (MLN) cells, especially T cells. Bovine LF or bovine serum albumin (control) was administered to mice once daily for 3 d. After 24 h from the last administration, IEL of the jejunum and ileum and MLN cells were isolated. These cells were cultured with and without the anti-T-cell-receptor antibody, and then the culture supernatants were assayed for cytokines with ELISA. Oral LF did not affect the ratio of T-cell subpopulations in IEL and MLN; however, LF enhanced both interferon (IFN)-gamma and interleukin (IL)-10 production by unstimulated IEL and by IEL stimulated with the alphabeta T-cell receptor but not with the gammadelta T-cell receptor. LF also enhanced both IFN-gamma and IL-10 production by stimulated and unstimulated MLN cells. The production level of IFN-gamma by MLN cells was correlated with that of IL-10. These results suggest that oral LF enhances the production of both Th1-type and Th2/Tr-type cytokines in the small intestine of healthy animals.
肠道黏膜免疫在黏膜和全身免疫反应中发挥重要作用。我们研究了口服牛乳铁蛋白（LF）对肠道上皮内淋巴细胞（IEL）和肠系膜淋巴结（MLN）细胞，尤其是T细胞产生细胞因子的影响。将牛LF或牛血清白蛋白（对照）每日给小鼠灌胃1次，共3天。末次给药后24小时，分离空肠和回肠的IEL以及MLN细胞。这些细胞在有或无抗T细胞受体抗体的情况下进行培养，然后用ELISA检测培养上清液中的细胞因子。口服LF不影响IEL和MLN中T细胞亚群的比例；然而，LF增强了未刺激的IEL以及由αβ T细胞受体而非γδ T细胞受体刺激的IEL产生的干扰素（IFN）-γ和白细胞介素（IL）-10。LF还增强了刺激和未刺激的MLN细胞产生的IFN-γ和IL-10。MLN细胞产生IFN-γ的水平与IL-10的水平相关。这些结果表明，口服LF可增强健康动物小肠中Th1型和Th2/Tr型细胞因子的产生。
