Blais Anne, Takakura Natsuko, Grauso Marta, Puel-Artero Caroline, Blachier François, Lan Annaïg
UMR-PNCA, Université Paris-Saclay, AgroParisTech, INRAE, 91120 Palaiseau, France.
Soredab, LDT, La Tremblaye, 78125 La Boissière-Ecole, France.
Nutrients. 2024 Nov 26;16(23):4040. doi: 10.3390/nu16234040.
BACKGROUND/OBJECTIVES: Injection of lipopolysaccharides (LPS) in experimental models induces a systemic inflammatory response that is associated with deleterious effects on intestinal morphology and physiology. In this study, we have studied in female mice the effects of dietary supplementation with bovine lactoferrin (bLF) given before intraperitoneal injection of LPS on jejunum and colon.
The first study evaluated the efficiency of different bLF and LPS concentrations to determine the optimal experimental conditions. For the second study mice were fed with 1% bLF before the LPS challenge (3 mg/kg body weight). Plasmatic markers of inflammation, intestinal morphology, permeability, and expression of genes related to epithelial differentiation, epithelial barrier function and intestinal inflammation in both small intestine and colon were evaluated.
bLF ingestion before the LPS challenge reduced the TNF-α circulating concentration, compared to control animals. This decrease in plasma TNF-α was correlated with improved intestinal permeability. The morphology of jejunal epithelium, which was affected by LPS challenge, was partly maintained by bLF. Measurement of the expression of genes encoding proteins involved in epithelial differentiation, intestinal inflammation, and epithelial barrier function suggests an overall protective effect of bLF against the adverse effects of LPS in the jejunum. In the colon, the effects of bLF ingestion on the subsequent LPS challenge, although protective, remain different when compared with those observed on jejunum.
Taken together, our data indicate that bLF dietary supplementation does have a protective effect on the deleterious intestinal alterations induced by LPS systemic inflammation.
背景/目的：在实验模型中注射脂多糖（LPS）会引发全身炎症反应，这与对肠道形态和生理的有害影响相关。在本研究中，我们在雌性小鼠中研究了在腹腔注射LPS之前给予牛乳铁蛋白（bLF）饮食补充对空肠和结肠的影响。
第一项研究评估了不同bLF和LPS浓度的效果，以确定最佳实验条件。第二项研究中，在LPS攻击（3mg/kg体重）前给小鼠喂食1%的bLF。评估了炎症的血浆标志物、肠道形态、通透性以及小肠和结肠中与上皮分化、上皮屏障功能和肠道炎症相关基因的表达。
与对照动物相比，在LPS攻击前摄入bLF可降低TNF-α的循环浓度。血浆TNF-α的这种降低与肠道通透性的改善相关。受LPS攻击影响的空肠上皮形态部分由bLF维持。对参与上皮分化、肠道炎症和上皮屏障功能的蛋白质编码基因表达的测量表明，bLF对LPS在空肠中的不良反应具有总体保护作用。在结肠中，摄入bLF对随后LPS攻击的影响虽然具有保护作用，但与在空肠中观察到的影响相比仍有所不同。
综上所述，我们的数据表明，bLF饮食补充对LPS全身炎症引起的有害肠道改变确实具有保护作用。
