使用微流控设备的活细胞折射测量法。
Live cell refractometry using microfluidic devices.
作者信息
Lue Niyom, Popescu Gabriel, Ikeda Takahiro, Dasari Ramachandra R, Badizadegan Kamran, Feld Michael S
机构信息
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
出版信息
Opt Lett. 2006 Sep 15;31(18):2759-61. doi: 10.1364/ol.31.002759.
Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.
我们使用希尔伯特相显微镜来提取定量相图像，测量了与培养中的活细胞相关的平均折射率。为了将细胞折射率和厚度对相位信号的贡献区分开来，我们将细胞限制在微通道中。通过与悬浮液中球形细胞的测量结果进行比较，证实了这些结果。
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