Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois.
Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois.
J Biophotonics. 2019 Dec;12(12):e201900178. doi: 10.1002/jbio.201900178. Epub 2019 Oct 3.
The development of three-dimensional (3D) cellular architectures during development and pathological processes involves intricate migratory patterns that are modulated by genetics and the surrounding microenvironment. The substrate composition of cell cultures has been demonstrated to influence growth, proliferation and migration in 2D. Here, we study the growth and dynamics of mouse embryonic fibroblast cultures patterned in a tissue sheet which then exhibits 3D growth. Using gradient light interference microscopy (GLIM), a label-free quantitative phase imaging approach, we explored the influence of geometry on cell growth patterns and rotational dynamics. We apply, for the first time to our knowledge, dispersion-relation phase spectroscopy (DPS) in polar coordinates to generate the radial and rotational cell mass-transport. Our data show that cells cultured on engineered substrates undergo rotational transport in a radially independent manner and exhibit faster vertical growth than the control, unpatterned cells. The use of GLIM and polar DPS provides a novel quantitative approach to studying the effects of spatially patterned substrates on cell motility and growth.
在发育和病理过程中，三维（3D）细胞结构的发展涉及复杂的迁移模式，这些模式受遗传和周围微环境的调节。细胞培养的基底组成已被证明会影响 2D 中的生长、增殖和迁移。在这里，我们研究了在组织片上进行图案设计的小鼠胚胎成纤维细胞培养物的生长和动力学，然后该组织片表现出 3D 生长。我们使用无标记定量相成像方法梯度光干涉显微镜 (GLIM) ，探索了几何形状对细胞生长模式和旋转动力学的影响。我们首次应用（据我们所知）在极坐标中进行的色散关系相光谱（DPS），以生成径向和旋转的细胞质量传递。我们的数据表明，在工程化基质上培养的细胞以径向独立的方式进行旋转运输，并且比对照的、无图案的细胞表现出更快的垂直生长。GLIM 和极 DPS 的使用为研究空间图案化基底对细胞迁移和生长的影响提供了一种新的定量方法。
