Lue Niyom, Choi Wonshik, Popescu Gabriel, Badizadegan Kamran, Dasari Ramachandra R, Feld Michael S
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Opt Express. 2008 Sep 29;16(20):16240-6. doi: 10.1364/oe.16.016240.
We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.
我们提出了一种无需物镜轴向扫描即可对处于平移运动中的活细胞进行三维成像的技术。利用聚焦光束照明获取细胞在横向平移连续点处的一组透射电场图像。基于惠更斯原理，从聚焦光束的电场图像合成角平面波。对于一组合成的角平面波，我们应用滤波反投影算法并获得活细胞折射率的三维图谱。我们将这种技术称为合成孔径层析相显微镜，它有可能与流式细胞术或微流控设备相结合，并能够从大量细胞中高通量获取定量折射率数据。
