Li Wei, Liu Su-Nan, Luo Duan-De, Zhao Lei, Zeng Ling-Lan, Zhang Shu-Ling, Li Shu-Li
Department of Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
World J Gastroenterol. 2006 Aug 14;12(30):4866-9. doi: 10.3748/wjg.v12.i30.4866.
To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC) in vitro, in order to find suitable resource of hepatocytes for bioartificial liver or liver transplantation.
The rat myeloid MSC was isolated and divided into three groups which were cultured by Friedensteion method, and then were induced by culture fluid, culture fluid plus cholestatic serum and culture fluid plus hepatocyte growth factor (HGF), respectively. Hepatocytoid cell as well as expression of CK18 and AFP was observed by immunohistochemistry.
After the induction for 21 d, hepatocytoid cell was observed, and its expression of CK18 and AFP was detected by immunohistochemistry in MSC cultured with cholestatic serum. Furthermore, on the 35th d, albumin mRNA was expressed in the cell, suggesting the inducing effect was similar to that by HGF.
Rat myeloid MSC can differentiate into hepatocyte lineage under appropriate condition. This method is easy to operate.
探讨髓系间充质干细胞（MSC）体外诱导分化为类肝细胞的能力，以寻找适用于生物人工肝或肝移植的肝细胞来源。
分离大鼠髓系MSC，采用Friedensteion法分为3组培养，分别用培养液、培养液加胆汁淤积血清、培养液加肝细胞生长因子（HGF）诱导。采用免疫组织化学法观察类肝细胞及细胞角蛋白18（CK18）和甲胎蛋白（AFP）的表达。
诱导21 d后，可见类肝细胞，用胆汁淤积血清培养的MSC经免疫组织化学检测到CK18和AFP表达。此外，第35 d时，细胞表达白蛋白mRNA，提示诱导效果与HGF相似。
大鼠髓系MSC在适当条件下可分化为肝细胞系。该方法操作简便。
