Emel'ianov V V
Mikrobiol Zh (1978). 1990 Jan-Feb;52(1):91-5.
A simple modification of the method for counting Rickettsiae is described. The Escherichia coli cells (ECC) which served as reference particles were stained in suspension with methylene blue mixed with Rickettsia prowazekii (RP) and quickly sprayed over the glass slide. After fixation the samples were stained according to the technique of Gimenez and examined in the light microscope under oil immersion. Through a grid in the eye-piece it was not so difficult to count red-coloured RP and dark-blue ECC against a background formed by impurities. To calculate RP concentration, the reference particles' concentration was multiplied by the dilution factor of RP suspension by the ratio of RP to ECC enumerated. The statistical approach has shown that the wash of the slides during staining procedure does not change this ratio. Differential staining of Rickettsiae with fuchsin is the main clue of this new method to count them even in the crude preparations of infected yolk sacs.
本文描述了一种用于计数立克次氏体方法的简单改进。用作参考颗粒的大肠杆菌细胞（ECC）在悬浮液中与普氏立克次氏体（RP）混合用亚甲蓝染色，然后迅速喷洒在载玻片上。固定后，样品按照吉姆尼兹染色技术进行染色，并在油镜下用光学显微镜检查。通过目镜中的网格，在由杂质形成的背景下，计数红色的RP和深蓝色的ECC并不困难。为了计算RP浓度，将参考颗粒的浓度乘以RP悬浮液的稀释倍数，再乘以所计数的RP与ECC的比例。统计方法表明，在染色过程中冲洗载玻片不会改变这个比例。用品红对立克次氏体进行鉴别染色是这种新方法的主要线索，即使在感染卵黄囊的粗制品中也能对它们进行计数。
