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用于对卵黄囊、组织培养提取物或纯化悬液中的立克次氏体进行计数的简单鉴别染色技术。

Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.

作者信息

Silverman D J, Fiset P, Wisseman C L

出版信息

J Clin Microbiol. 1979 Mar;9(3):437-40. doi: 10.1128/jcm.9.3.437-440.1979.

DOI:10.1128/jcm.9.3.437-440.1979
PMID:88460
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273043/
Abstract

A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle.

摘要

描述了一种鉴别染色方法，该方法使用吖啶橙染色的立克次氏体和番红染色的痢疾志贺氏菌标准悬液，用于对生长周期不同阶段收获的立克次氏体进行计数。

相似文献

1
Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.用于对卵黄囊、组织培养提取物或纯化悬液中的立克次氏体进行计数的简单鉴别染色技术。
J Clin Microbiol. 1979 Mar;9(3):437-40. doi: 10.1128/jcm.9.3.437-440.1979.
2
[A simple method for counting Rickettsia cells].[一种计数立克次氏体细胞的简单方法]
Mikrobiol Zh (1978). 1990 Jan-Feb;52(1):91-5.
3
Studies of the rickettsial plaque assay technique.立克次氏体蚀斑测定技术的研究
Infect Immun. 1972 May;5(5):715-22. doi: 10.1128/iai.5.5.715-722.1972.
4
ELECTRON MICROSCOPIC OBSERVATIONS OF THE DEVELOPMENT OF COXIELLA BURNETII IN THE CHICK YOLK SAC.鸡卵黄囊内伯氏考克斯体发育的电子显微镜观察
J Bacteriol. 1964 Oct;88(4):1130-8. doi: 10.1128/jb.88.4.1130-1138.1964.
5
Methods for purification of Rickettsia prowazekii separated from the host tissue: a step-by-step comparison.从宿主组织中分离普氏立克次体的纯化方法：逐步比较
Acta Virol. 1989 Aug;33(4):361-70.
6
The use of DNase for preparation of host tissue DNA-free rickettsial suspensions.使用脱氧核糖核酸酶制备不含宿主组织DNA的立克次氏体悬浮液。
Acta Virol. 1984 Jan;28(1):73-7.
7
Method for counting Rickettsiae and Chlamydiae in purified suspensions.纯化悬浮液中立克次氏体和衣原体的计数方法。
J Bacteriol. 1968 Jan;95(1):259-61. doi: 10.1128/jb.95.1.259-261.1968.
8
Phospholipid composition of Rickettsia prowazeki grown in chicken embryo yolk sacs.在鸡胚卵黄囊中生长的普氏立克次体的磷脂组成。
J Bacteriol. 1978 Oct;136(1):175-8. doi: 10.1128/jb.136.1.175-178.1978.
9
STAINING RICKETTSIAE IN YOLK-SAC CULTURES.卵黄囊培养物中里克次氏体的染色
Stain Technol. 1964 May;39:135-40. doi: 10.3109/10520296409061219.
10
[ELECTRON MICROSCOPIC STUDIES ON RICKETTSIA PROWAZEKII IN ULTRATHIN SECTIONS OF THE YOLK SAC].[在卵黄囊超薄切片中对普氏立克次体的电子显微镜研究]
Mikrobiol Zh. 1964;26:58-63.

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Differentiation between virulent and avirulent strains of Rickettsia prowazekii by macrophage-like cell lines.通过巨噬细胞样细胞系区分普氏立克次体的强毒株和无毒株。
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Infect Immun. 1982 Sep;37(3):1066-73. doi: 10.1128/iai.37.3.1066-1073.1982.

本文引用的文献

1
Method for counting Rickettsiae and Chlamydiae in purified suspensions.纯化悬浮液中立克次氏体和衣原体的计数方法。
J Bacteriol. 1968 Jan;95(1):259-61. doi: 10.1128/jb.95.1.259-261.1968.
2
Effects of various suspending media on plaque formation by rickettsiae in tissue culture.不同悬浮介质对立克次氏体在组织培养中形成菌斑的影响。
Infect Immun. 1972 Oct;6(4):550-6. doi: 10.1128/iai.6.4.550-556.1972.
3
Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation.通过泛影葡胺密度梯度离心法从卵黄囊和L细胞宿主成分中分离出活的鼠型斑疹伤寒立克次体。
Appl Microbiol. 1975 Sep;30(3):456-63. doi: 10.1128/am.30.3.456-463.1975.
4
In vitro studies on rickettsia-host cell interactions: intracellular growth cycle of virulent and attenuated Rickettsia prowazeki in chicken embryo cells in slide chamber cultures.立克次氏体与宿主细胞相互作用的体外研究：在玻片培养室培养的鸡胚细胞中，强毒株和减毒株普氏立克次氏体的细胞内生长周期
Infect Immun. 1975 Jun;11(6):1391-404. doi: 10.1128/iai.11.6.1391-1401.1975.
5
Limits of rickettsial infectivity.立克次氏体感染性的限度。
Infect Immun. 1978 Jan;19(1):239-45. doi: 10.1128/iai.19.1.239-245.1978.
6
In vitro studies on Rickettsia-host cell interactions: lag phase in intracellular growth cycle as a function of stage of growth of infecting Rickettsia prowazeki, with preliminary observations on inhibition of rickettsial uptake by host cell fragments.立克次氏体与宿主细胞相互作用的体外研究：细胞内生长周期中的延迟期与感染的普氏立克次氏体生长阶段的关系，以及关于宿主细胞碎片抑制立克次氏体摄取的初步观察。
Infect Immun. 1976 Jun;13(6):1749-60. doi: 10.1128/iai.13.6.1749-1760.1976.