Kekcheeva N G, Kokorin I N, Popov V L, Chereshkova E A, Smirnova N S, Vovk O A, Shirokova E M
N.F. Gamaleya Research Institute of Epidemiology and Microbiology, AMS USSR, Moscow.
Acta Virol. 1992 Jan;36(1):103-10.
Rickettsia prowazekii is able to multiply and persist for a long time in cotton rat macrophage culture (29-days observation period). Electron microscopic studies showed that the structure of Rickettsiae remained intact at different intervals post-inoculation (p.i.). In the course of persistence Rickettsiae revealed a reduced capacity to infect chick embryos and guinea pigs, however, the infectious agent could be isolated at all stages of persistence of cultured cells such as fibroblasts of the guinea pig embryo, macrophages of intact cotton rats.
普氏立克次体能够在棉鼠巨噬细胞培养物中繁殖并长时间存活（观察期为29天）。电子显微镜研究表明，接种后不同时间段，立克次体的结构保持完整。在持续存在的过程中，立克次体感染鸡胚和豚鼠的能力有所下降，然而，在培养细胞（如豚鼠胚胎成纤维细胞、健康棉鼠的巨噬细胞）持续存在的各个阶段都能分离到感染因子。
