Dalma-Weiszhausz Dennise D, Warrington Janet, Tanimoto Eugene Y, Miyada C Garrett
Expression Product Development, AFFY-METRIX, INC., Santa Clara, California, USA.
Methods Enzymol. 2006;410:3-28. doi: 10.1016/S0076-6879(06)10001-4.
The intent of this chapter is to provide the reader with a review of GeneChip technology and the complete system it represents, including its versatility, components, and the exciting applications that are enabled by this platform. The following aspects of the technology are reviewed: array design and manufacturing, target preparation, instrumentation, data analysis, and both current and future applications. There are key differentiators between Affymetrix' GeneChip technology and other microarray-based methods. The most distinguishing feature of GeneChip microarrays is that their manufacture is directed by photochemical synthesis. Because of this manufacturing technology, more than a million different probes can be synthesized on an array roughly the size of a thumbnail. These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation. Over the years the GeneChip platform has proven to be a reliable and robust system, enabling many new discoveries and breakthroughs to be made by the scientific community.
本章旨在为读者回顾基因芯片技术及其所代表的完整系统，包括其多功能性、组件以及该平台所带来的令人兴奋的应用。将对该技术的以下方面进行回顾：阵列设计与制造、靶标制备、仪器设备、数据分析以及当前和未来的应用。Affymetrix的基因芯片技术与其他基于微阵列的方法存在关键差异。基因芯片微阵列最显著的特点是其制造由光化学合成引导。由于这种制造技术，可以在一块大致 thumbnail大小的阵列上合成超过一百万个不同的探针。这些数量使得能够包含多个探针来检测相同的靶标序列，为数据解读提供了统计学上的严谨性。多年来，基因芯片平台已被证明是一个可靠且强大的系统，使科学界能够取得许多新的发现和突破。
