Gnepp D R, Wick M R
St. Louis University School of Medicine, Department of Pathology and Otolaryngology--Head and Neck Surgery, MO.
Cancer. 1990 Jul 1;66(1):185-92. doi: 10.1002/1097-0142(19900701)66:1<185::aid-cncr2820660133>3.0.co;2-4.
Small cell carcinomas of the major salivary glands are rare tumors, accounting for less than 1% of malignant neoplasms at these sites. To date, approximately 41 such tumors have been described. They recently have been classified into two groups, based on the ultrastructural presence or absence of intracytoplasmic neuroendocrine (NE) granules, "small cell neuroendocrine carcinoma" and "small cell ductal carcinoma". This study concerns 11 primary small cell carcinomas that had been previously studied ultrastructurally; it was undertaken to determine whether these lesions possessed a neuroendocrine phenotype, using a battery of immunohistochemical stains. Antibodies to epithelial membrane antigen (EMA), cytokeratin (CK), Leu 7, vimentin (VIM), synaptophysin (SYN), chromogranin (CHR), and neuron-specific enolase (NSE) were employed, with the avidin-biotin-peroxidase complex technique and paraffin sections. All tumors in this study expressed at least one neuroendocrine marker. In eight tumors EMA was found; CK was present in all 11 cases, seven of which demonstrated focal paranuclear staining. Leu 7 was seen in eight tumors, VIM was expressed in two cases, SYN was found in three tumors, and CHR was detected in three neoplasms. Anti-neuron-specific enolase labeled eight tumors. From the preceding data one may conclude that all small cell salivary gland carcinomas have neuroendocrine characteristics, even though dense core granules cannot be demonstrated in some of them ultrastructurally.
大唾液腺小细胞癌是罕见肿瘤，占这些部位恶性肿瘤的比例不到1%。迄今为止，已报道约41例此类肿瘤。最近，根据超微结构中是否存在胞质内神经内分泌（NE）颗粒，将它们分为两组，即“小细胞神经内分泌癌”和“小细胞导管癌”。本研究涉及11例先前已进行超微结构研究的原发性小细胞癌；旨在通过一系列免疫组织化学染色来确定这些病变是否具有神经内分泌表型。采用抗上皮膜抗原（EMA）、细胞角蛋白（CK）、Leu 7、波形蛋白（VIM）、突触素（SYN）、嗜铬粒蛋白（CHR）和神经元特异性烯醇化酶（NSE）的抗体，运用抗生物素蛋白-生物素-过氧化物酶复合物技术和石蜡切片。本研究中的所有肿瘤均表达至少一种神经内分泌标志物。在8例肿瘤中发现EMA；11例病例均存在CK，其中7例显示局灶性核旁染色。在8例肿瘤中可见Leu 7，2例表达VIM，3例肿瘤中发现SYN，3例肿瘤中检测到CHR。抗神经元特异性烯醇化酶标记了8例肿瘤。根据上述数据可以得出结论，所有小细胞唾液腺癌均具有神经内分泌特征，即使其中一些在超微结构中无法显示致密核心颗粒。
