1型单纯疱疹病毒温度敏感突变体的DNA合成及DNA聚合酶活性
DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants.
作者信息
Aron G M, Purifoy D J, Schaffer P A
出版信息
J Virol. 1975 Sep;16(3):498-507. doi: 10.1128/JVI.16.3.498-507.1975.
Abstract
Fifteen temperature-sensitive mutants of herpes simplex virus type 1 were studied with regard to the relationship between their ability to synthesize viral DNA and to induce viral DNA polymerase (DP) activity at permissive (34 C) and nonpermissive (39 C) temperatures. At 34 C, all mutants synthesized viral DNA, while at 39 C four mutants demonstrated a DNA+ phenotype, three were DNA+/-, and eight were DNA-. DNA+ mutants induced levels of DP activity similar to thhose of the wild-type virus at both temperatures, and DNA+/- mutants induced reduced levels of DP activity at 39 C but not at 34 C. Among the DNA- mutants three were DP+, two were DP+/-, and three showed reduced DP activity at 34 C with no DP activity at 39 C. DNA-, DP- mutants induced the synthesis of a temperature-sensitive DP as determined by in vivo studies.
摘要
对15株1型单纯疱疹病毒温度敏感突变体进行了研究，以探讨它们在允许温度（34℃）和非允许温度（39℃）下合成病毒DNA的能力与诱导病毒DNA聚合酶（DP）活性之间的关系。在34℃时，所有突变体均能合成病毒DNA，而在39℃时，4个突变体表现为DNA+表型，3个为DNA+/-，8个为DNA-。DNA+突变体在两个温度下诱导的DP活性水平与野生型病毒相似，DNA+/-突变体在39℃时诱导的DP活性水平降低，但在34℃时未降低。在DNA-突变体中，3个为DP+，2个为DP+/-，3个在34℃时DP活性降低，在39℃时无DP活性。通过体内研究确定，DNA-、DP-突变体诱导合成了一种温度敏感的DP。
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