Liu Yi, Chen Hu-Biao, Zhao Yu-Ying, Wang Bin, Zhang Qing-Ying, Zhang Lei, Tu Pengfei
Department of Natural Medicines and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100083, PR China.
J Agric Food Chem. 2006 Sep 6;54(18):6634-9. doi: 10.1021/jf061335o.
Radix hedysari is not only a restorative food but also a famous Traditional Chinese Medicine. In this study, a simple, reliable, and reproducible high-performance liquid chromatography-ultraviolet method was developed for the first time to determine the true contents of five major flavoniods, naringenin-7-O-alpha-L-rhamanopyranosyl-(1-->2)-beta-d-glucopyranoside, ononin, formononetin, medicarpin, and an unstable flavonoid malonate (formononetin-7-O-beta-D-glucoside-6' '-O-malonate, FGM), and the stability of FGM was also investigated. Flavoniods were selected as chemical markers because they have appeared as one of the major bioactive compound groups in Radix hedysari. The stability results of FGM at different pH showed that it remained relatively stable in acidic aqueous methanol of pH 3. The HPLC assay with an improved sample preparation scheme can be readily utilized as a suitable "chemical" quality control method for Radix hedysari.
红芪不仅是一种滋补食品，也是一种著名的中药材。在本研究中，首次建立了一种简单、可靠且可重复的高效液相色谱 - 紫外检测方法，用于测定五种主要黄酮类化合物，即柚皮素 -7-O-α-L-鼠李糖基-(1→2)-β-D-葡萄糖苷、芒柄花苷、芒柄花素、美迪紫檀素以及一种不稳定的黄酮丙二酸酯（芒柄花素 -7-O-β-D-葡萄糖苷-6''-O-丙二酸酯，FGM）的真实含量，同时还研究了FGM的稳定性。选择黄酮类化合物作为化学标志物，因为它们是红芪中主要的生物活性化合物组之一。FGM在不同pH值下的稳定性结果表明，它在pH 3的酸性甲醇水溶液中相对稳定。采用改进样品制备方案的高效液相色谱分析方法可方便地用作红芪合适的“化学”质量控制方法。
