Vollmer G, Siegal G P, Chiquet-Ehrismann R, Lightner V A, Arnholdt H, Knuppen R
Institut für Biochemische Endokrinologie, Medizinische Universität, Lübeck, Federal Republic of Germany.
Lab Invest. 1990 Jun;62(6):725-30.
To investigate the involvement of tenascin, an extracellular matrix glycoprotein, in epithelial growth and malignancy, its specific distribution pattern in the human uterus was examined immunohistochemically. During the proliferative phase of the menstrual cycle, this antigen was found as a sharp band around the endometrial glands. The immunoreactivity persisted until the early postovulatory phase of the menstrual cycle, but was not detectable in the glandular or stromal compartment during this later secretory stage, instead endometrial arterioles were immunostained. In marked contradistinction, when antibodies directed against tenascin were applied to sections of endometrial adenocarcinoma, almost the entire extracellular space stained, whereas the neoplastic cells themselves were nonreactive, whatever the degree of tumor differentiation. In precancerous proliferative lesions of the endometrium, tenascin's presence was variable. It was detectable around some superficial glands demonstrating cystic hyperplasia and around all deeply situated glands at the endometrial/myometrial interface. In cases of adenomatous hyperplasia, tenascin immunolocalized throughout the extracellular space of the stroma and the staining intensity was increased as the hyperplasia became more atypical. We therefore conclude that tenascin may be a stromal marker for epithelial proliferative states including those associated with malignancies of the endometrium.
为了研究细胞外基质糖蛋白肌腱蛋白在上皮生长和恶性肿瘤中的作用，采用免疫组织化学方法检测了其在人子宫中的特异性分布模式。在月经周期的增殖期，该抗原表现为围绕子宫内膜腺体的一条清晰条带。这种免疫反应性持续到月经周期的排卵后早期，但在随后的分泌期，在腺体或基质部分未检测到，相反，子宫内膜小动脉被免疫染色。与之形成显著对比的是，当将针对肌腱蛋白的抗体应用于子宫内膜腺癌切片时，几乎整个细胞外间隙都被染色，而肿瘤细胞本身无论肿瘤分化程度如何均无反应。在子宫内膜癌前增殖性病变中，肌腱蛋白的存在情况各不相同。在一些表现为囊性增生的浅表腺体周围以及子宫内膜/肌层界面所有深部腺体周围均可检测到。在腺瘤样增生病例中，肌腱蛋白免疫定位在整个基质的细胞外间隙，并且随着增生变得更不典型，染色强度增加。因此，我们得出结论，肌腱蛋白可能是上皮增殖状态的一种基质标志物，包括与子宫内膜恶性肿瘤相关的状态。
