Tiitta O, Sipponen P, Gould V, Virtanen I
Department of Anatomy, University of Helsinki, Finland.
Virchows Arch. 1994;425(4):369-74. doi: 10.1007/BF00189574.
We studied the expression of tenascin (Tn) in human stomach. In the normal mucosa of the antrum and body, Tn reaction was only seen in the muscularis mucosae, in the region of the pyloric sphincter and in the duodenum, a Tn-immunoreactive rim was seen underlying surface epithelial cells. Antral gastritis, irrespective of the degree of inflammation, showed a rim-like Tn expression under the surface epithelial cells but no Tn reaction was seen in mild chronic gastritis of the body. In some moderate and severe examples of chronic gastritis a delicate Tn-reactive line was seen to underline the surface epithelium focally and the neck regions of gastric pits. Discontinuous Tn immunoreactivity was sometimes seen beneath hyperplastic epithelium in both parts of the stomach. A Tn-immunoreactive line was seldom seen surrounding glands showing intestinal metaplasia. In both benign and malignant ulcers prominent Tn immunoreaction was seen at the base of ulcers extending deep into the underlying muscularis. Only severely dysplastic lesions displayed Tn in the lamina propria, in close association with capillaries. In early forms of diffuse gastric cancer (DGCA) raggedly increased Tn staining was seen in the lamina propria underlying affected surface epithelial cells. In advanced forms of DGCA consistent Tn expression was seen in the tumour stroma. A distinct Tn reaction was seen surrounding invasive tumour cell nests of intestinal type gastric cancer (IGCA) in the submucosa, whereas in early forms of the tumour enhanced Tn reaction was noted predominantly in the upper part of the lamina propria in the vicinity of dysplastic elements. Notably, while most invading DGCA tumour cell nests showed no Tn in the submucosa and muscle cell layer, invading IGCA islets showed prominent expression of Tn. The most conspicuous Tn enhancement in the stomach is seen in invasive tumours and in ulcers suggesting that Tn is an important stromal component in malignant growth and in lesions undergoing active repair and remodelling.
我们研究了腱生蛋白（Tn）在人胃中的表达。在胃窦和胃体的正常黏膜中，Tn反应仅见于黏膜肌层、幽门括约肌区域以及十二指肠，在表面上皮细胞下方可见一条Tn免疫反应性边缘。胃窦炎，无论炎症程度如何，表面上皮细胞下方均呈现边缘样Tn表达，但胃体轻度慢性胃炎未见Tn反应。在一些中度和重度慢性胃炎病例中，可见一条细微的Tn反应线局部位于表面上皮和胃小凹颈部区域下方。在胃的两部分增生上皮下方有时可见不连续的Tn免疫反应性。很少见到围绕肠化生腺体的Tn免疫反应性线。在良性和恶性溃疡的底部均可见到突出的Tn免疫反应，溃疡深入至下方的肌层。只有重度发育异常病变在固有层中显示Tn，且与毛细血管密切相关。在弥漫性胃癌（DGCA）的早期形式中，受累表面上皮细胞下方的固有层中可见Tn染色不规则增加。在DGCA的晚期形式中，肿瘤基质中可见一致的Tn表达。在黏膜下层，可见明显的Tn反应围绕肠型胃癌（IGCA）的浸润性肿瘤细胞巢，而在肿瘤的早期形式中，主要在发育异常区域附近的固有层上部观察到增强的Tn反应。值得注意的是，虽然大多数浸润性DGCA肿瘤细胞巢在黏膜下层和肌层细胞层中未显示Tn，但浸润性IGCA小岛显示出突出的Tn表达。胃中最明显的Tn增强见于浸润性肿瘤和溃疡，提示Tn是恶性生长以及正在进行积极修复和重塑的病变中的一种重要基质成分。
