枯草芽孢杆菌168重组菌株对伊枯草菌素A的生物膜发酵
Biofilm fermentation of iturin A by a recombinant strain of Bacillus subtilis 168.
作者信息
Rahman Mohammad Shahedur, Ano Takashi, Shoda Makoto
机构信息
Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
出版信息
J Biotechnol. 2007 Jan 10;127(3):503-7. doi: 10.1016/j.jbiotec.2006.07.013. Epub 2006 Jul 27.
Bacillus subtilis 168 produces thin and fragile biofilm in the static culture, however, it was found out that its transformant B. subtilis RM/iSd16 containing wild sfp, itu operon and degQ, which produced lipopeptide antibiotic iturin A, produced thick and much stable biofilm. Production of iturin A by RM/iSd16 in biofilm was almost two times higher compared to that in the submerged culture at 28 degrees C.
枯草芽孢杆菌168在静置培养时会产生薄且易碎的生物膜，然而，研究发现其含有野生型sfp、itu操纵子和degQ的转化体枯草芽孢杆菌RM/iSd16，该菌株能产生脂肽抗生素伊枯草菌素A，可形成厚且更稳定的生物膜。在28摄氏度下，RM/iSd16在生物膜中产生的伊枯草菌素A几乎是其在深层培养中产量的两倍。
Zotero 插件