Bartholdi M F
Cellular and Molecular Biology Group, Los Alamos National Laboratory, N. Mex.
Pathobiology. 1990;58(2):118-28. doi: 10.1159/000163573.
Applications of flow karyotype analysis and flow chromosome sorting are being developed and are becoming increasingly relevant to clinical research. Among these applications are the quantitative analysis of chromosomal DNA content changes enabling the detection of minute deletions or unbalanced translocations, the quantitative analysis of aneuploidy for trisomy screening, and the sorting of derivative chromosomes for mapping chromosome breakpoints. Flow karyotyping has been accomplished on samples with clinical relevance including peripheral blood from both children and cancer patients as well as amniotic cell cultures. New techniques in flow cytogenetics to resolve chromosomes by morphology or by DNA sequence hybridizations are assessed for their promise. Although flow karyotyping has not yet developed into an 'automated' karyotyping technique, when used in conjunction with classical cytogenetic techniques, new information of significance to the clinician can be produced.
流式核型分析和流式染色体分选的应用正在不断发展，并且在临床研究中的相关性日益增强。这些应用包括对染色体DNA含量变化进行定量分析以检测微小缺失或不平衡易位、对非整倍体进行定量分析以进行三体筛查，以及对衍生染色体进行分选以绘制染色体断点图谱。流式核型分析已在具有临床相关性的样本上得以实现，这些样本包括儿童和癌症患者的外周血以及羊水细胞培养物。评估了通过形态学或DNA序列杂交解析染色体的流式细胞遗传学新技术的前景。尽管流式核型分析尚未发展成为一种“自动化”核型分析技术，但与经典细胞遗传学技术结合使用时，可为临床医生提供具有重要意义的新信息。
