Mooren F C, Lechtermann A, Fobker M, Brandt B, Sorg C, Völker K, Nacken W
Institute of Sports Medicine, Muenster University Hospital, Horstmarer Landweg 39, 48129 Muenster, Germany.
Int J Sports Med. 2006 Sep;27(9):751-8. doi: 10.1055/s-2005-872909.
Exercise shares many similarities with the acute phase response of inflammatory diseases. Recently, elevated serum levels of the novel pro-inflammatory molecules of the S100 protein family, S100A8 and S100A9, have been associated with various inflammatory diseases. The present study was conducted to assess their potential roles as inflammatory markers in monitoring the exercise-induced immune response. Seventeen male subjects of different training status performed a marathon run. Furthermore 13 subjects (10 male, 3 female) performed three different treadmill tests: strenuous (STE), moderate (MTE), and downhill (DTE). S100A8/A9 complexes were measured by ELISA, while white blood cell count (WBC) and C-reactive protein (CRP) were used as markers of the inflammatory response. Serum creatine kinase (CK) concentration was determined as a marker for muscle damage. After marathon S100A8/A9 increased dramatically during the early post-exercise period and returned to resting levels one day after the run. A similar pattern was found for WBC, while CK and CRP reached their maximum on the day after the run. Moreover, S100A8/A9 release was higher in the subgroup of well-trained athletes. The kinetic of the S100A8/A9 release after the treadmill tests depended on exercise intensity and was prolonged after eccentric exercise. In summary, the present results indicate that the novel pro-inflammatory molecules S100A8/A9 are very early and sensitive markers of the exercise-induced inflammatory response. Further investigations are necessary to evaluate the applicability of S100A8/A9 for monitoring the training process and to elucidate the dependence on training status.
运动与炎症性疾病的急性期反应有许多相似之处。最近，血清中新型促炎分子S100蛋白家族的S100A8和S100A9水平升高与各种炎症性疾病有关。本研究旨在评估它们作为炎症标志物在监测运动诱导的免疫反应中的潜在作用。17名不同训练状态的男性受试者进行了马拉松跑步。此外，13名受试者（10名男性，3名女性）进行了三种不同的跑步机测试：剧烈运动（STE）、中等强度运动（MTE）和下坡运动（DTE）。通过酶联免疫吸附测定法（ELISA）测量S100A8/A9复合物，同时使用白细胞计数（WBC）和C反应蛋白（CRP）作为炎症反应的标志物。测定血清肌酸激酶（CK）浓度作为肌肉损伤的标志物。马拉松赛后，S100A8/A9在运动后早期急剧增加，并在跑步一天后恢复到静息水平。白细胞计数（WBC）也呈现类似模式，而CK和CRP在跑步后一天达到峰值。此外，训练有素的运动员亚组中S100A8/A9的释放更高。跑步机测试后S100A8/A9释放的动力学取决于运动强度，离心运动后延长。总之，目前的结果表明，新型促炎分子S100A8/A9是运动诱导的炎症反应的非常早期和敏感的标志物。有必要进一步研究以评估S100A8/A9在监测训练过程中的适用性，并阐明其对训练状态的依赖性。
