抗大鼠肾脏γ-谷氨酰转移酶单克隆抗体的制备、交叉反应性及表位分析
Production, cross reactivity, and epitope analysis of monoclonal antibodies against rat kidney gamma glutamyltransferase.
作者信息
Bayad J, Sabolovic N, Bagrel D, Visvikis A, Wellman M, Siest G
机构信息
Centre du Médicament, URA CNRS 597, Faculté des Sciences Pharmaceutiques et Biologiques, Nancy, France.
出版信息
Cell Biol Toxicol. 1990 Apr;6(2):157-70. doi: 10.1007/BF00249591.
Eighteen IgG1 monoclonal antibodies (Mabs) have been produced against gamma-glutamyl transferase (GGT) from rat kidney. They were specific to the light subunit of the enzyme with affinity constants ranging from 0.3 to 7.5 10(8) M-1, while they did not react with GGT from other sources i.e. human and pig kidney, rat and guinea pig liver, suggesting species and organ specificity. Two of the Mabs (No 11 and 21) lost their immunoreactivities towards rat kidney GGT in the presence of N-acetyl-neuraminic acid, while immunoreactivities of the other Mabs were unchanged. Furthermore, Mabs No 11 and 21 did not react with desialylated rat kidney GGT. These findings suggest that N-acetyl-neuraminic acid is involved in the epitopes recognized by these two Mabs.
已制备出18种针对大鼠肾脏γ-谷氨酰转移酶（GGT）的IgG1单克隆抗体（Mab）。它们对该酶的轻亚基具有特异性，亲和常数范围为0.3至7.5×10⁸ M⁻¹，而它们不与其他来源的GGT反应，即人及猪肾脏、大鼠及豚鼠肝脏中的GGT，表明具有物种和器官特异性。其中两种单克隆抗体（11号和21号）在N-乙酰神经氨酸存在下对大鼠肾脏GGT失去免疫反应性，而其他单克隆抗体的免疫反应性未改变。此外，11号和21号单克隆抗体不与去唾液酸化的大鼠肾脏GGT反应。这些发现表明N-乙酰神经氨酸参与了这两种单克隆抗体识别的表位。
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