Qureshi Imtiaz Ahmad, Jamal Shahid, Mamoon Nadira, Luqman Muhammad, Sharif Muhammad Ashraf
Department of Histopathology, Armed Forces Institutes of Pathology (AFIP), Rawalpindi.
J Coll Physicians Surg Pak. 2006 Sep;16(9):590-3.
To evaluate accuracy of intraoperative crush smears diagnosis of neurosurgical biopsies.
Cross-sectional study.
The Armed Forces Institute of Pathology, Rawalpindi, from February 2002 to February 2003.
One hundred, neurosurgical biopsies were received for intraoperative consultation over a period of one year. Clinical information regarding age, gender, history, and CT scan or MRI findings were recorded. Crush smears were prepared, fixed in 95% alcohol and stained with rapid haemotoxylin and eosin (H&E) stains. Diagnosis was categorized into inflammatory, benign and malignant tumours. Remaining tissue was fixed overnight in 10% formalin for histological sections. Permanent H&E sections were used as the gold standard. The average time required for intraoperative cytological diagnosis was 8 minutes.
Out of 100 neurosurgical biopsies, crush smears of 94 were considered suitable for interpretation. There were 8 inflammatory, 41 benign and 41 malignant tumours, including 4 inconclusive. Eightyfour of the crush smears diagnosis agreed with the histological diagnosis. Overall diagnostic accuracy of crush smears was 93.3%. Diagnosis of inflammatory, benign and malignant tumours showed specificity of 98.7%, 96% and 94%, and a sensitivity of 70%, 97.5% and 95% respectively.
Crush smears are useful in the intraoperative diagnosis of space occupying lesions of central nervous system. The crush smears cytology was found highly reliable, rapid and inexpensive mode of intraoperative diagnosis.
评估神经外科活检术中压片涂片诊断的准确性。
横断面研究。
2002年2月至2003年2月，拉瓦尔品第武装部队病理研究所。
一年内接收了100例用于术中会诊的神经外科活检样本。记录了有关年龄、性别、病史以及CT扫描或MRI检查结果的临床信息。制备压片涂片，用95%酒精固定，并用快速苏木精和伊红（H&E）染色。诊断分为炎症性、良性和恶性肿瘤。剩余组织在10%福尔马林中固定过夜以制作组织切片。永久性H&E切片用作金标准。术中细胞学诊断所需的平均时间为8分钟。
100例神经外科活检样本中，94例的压片涂片被认为适合解读。其中有8例炎症性病变、41例良性肿瘤和41例恶性肿瘤，包括4例无法确诊的病例。84例压片涂片诊断与组织学诊断一致。压片涂片的总体诊断准确率为93.3%。炎症性、良性和恶性肿瘤的诊断特异性分别为98.7%、96%和94%，敏感性分别为70%、97.5%和95%。
压片涂片在中枢神经系统占位性病变的术中诊断中有用。压片涂片细胞学检查是一种高度可靠、快速且廉价的术中诊断方法。
