Kar Mousumi, Sengupta Moumita, Sarkar Saurav, Bera Saikat, Datta Chhanda, Chatterjee Uttara, Ghosh Samarendra Nath
Department of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.
Department of Neurosurgery, Bangur Institute of Neurology, Kolkata, West Bengal, India.
J Cytol. 2018 Jul-Sep;35(3):139-142. doi: 10.4103/JOC.JOC_11_17.
Squash cytology for intra operative diagnosis of central nervous system (CNS) tumors is an immensely important modality. Though its role in brain lesions is unquestionable and has been proven in a number of studies, its utility for spinal lesions is still a grey zone.
To assess the diagnostic accuracy of squash preparation in spinal lesions and its statistical significance (sensitivity, specificity, positive predictive value, negative predictive value) following histological confirmation.
A total of 57 cases of spinal tumors were taken. May-Grunewald-Giemsa staining (MGG) and Hematoxylin-Eosin (H&E) were done in each one of them. Rest of the tissue was processed for histological diagnosis and results were compared.
In our study, histology was taken as the gold standard. By comparing the results, squash preparation had sensitivity of 95.75%, specificity 80.0%, positive predictive value (PPV)95.74%, and negative predictive value (NPV) 80.80%. Schwannoma was found to be the most prevalent tumor in the spine (17/57) in our study, followed by meningioma (13/57). Diagnostic accuracy for schwannoma was fairly high i.e. 92.3%, followed by meningioma (82.35%). Highest diagnostic accuracy was documented in intradural, extramedullary compartment.
Inspite of having pitfalls and various limitations in case of spinal lesions, squash preparation is a rapid and easy method with fairly high diagnostic accuracy. So it can be reliably used as an intraoperative diagnostic tool in spinal lesions.
用于中枢神经系统（CNS）肿瘤术中诊断的 squash 细胞学检查是一种极为重要的方法。尽管其在脑病变中的作用毋庸置疑，并且已在多项研究中得到证实，但其在脊柱病变中的效用仍存在争议。
评估 squash 制片在脊柱病变中的诊断准确性及其在组织学确诊后的统计学意义（敏感性、特异性、阳性预测值、阴性预测值）。
共纳入 57 例脊柱肿瘤病例。对每例病例均进行 May-Grunewald-Giemsa 染色（MGG）和苏木精-伊红（H&E）染色。其余组织进行组织学诊断，并比较结果。
在我们的研究中，组织学被视为金标准。通过比较结果，squash 制片的敏感性为 95.75%，特异性为 80.0%，阳性预测值（PPV）为 95.74%，阴性预测值（NPV）为 80.80%。在我们的研究中，神经鞘瘤是脊柱中最常见的肿瘤（17/57），其次是脑膜瘤（13/57）。神经鞘瘤的诊断准确性相当高，即 92.3%，其次是脑膜瘤（82.35%）。硬膜内、髓外间隙的诊断准确性最高。
尽管在脊柱病变中存在缺陷和各种局限性，但 squash 制片是一种快速简便的方法，诊断准确性相当高。因此，它可以可靠地用作脊柱病变的术中诊断工具。
