Fixler Dror, Garcia Javier, Zalevsky Zeev, Weiss Aryeh, Deutsch Mordechai
School of Engineering, Bar-Ilan University, Ramat-Gan 52900, Israel.
Micron. 2007;38(2):121-8. doi: 10.1016/j.micron.2006.07.008. Epub 2006 Jul 28.
In this manuscript we present a novel super resolving approach based upon projection of a random speckle pattern onto samples observed through a microscope. The projection of the speckle pattern is created by coherent illumination of the inspected pattern through a diffuser. Due to local interference of the coherent wave front with itself, a random speckle pattern is superimposed on the sample. This speckle pattern can be scanned over the object. A super-resolved image can be extracted from a temporal sequence of images by appropriate digital processing of the image stream. The resulting resolution is significantly higher than the diffraction limitation of the microscope objective. The new super-resolution method is demonstrated by application to fluorescence of biological samples.
在本论文中，我们提出了一种基于将随机散斑图案投影到通过显微镜观察的样本上的新型超分辨率方法。散斑图案的投影是通过漫射器对被检查图案进行相干照明而产生的。由于相干波前自身的局部干涉，随机散斑图案叠加在样本上。该散斑图案可以在物体上进行扫描。通过对图像流进行适当的数字处理，可以从图像的时间序列中提取超分辨率图像。所得分辨率明显高于显微镜物镜的衍射极限。通过将其应用于生物样本的荧光，证明了这种新的超分辨率方法。
